doi: 10.1016/0076-6879(86)28113-6.
Molecular cloning of bovine LDL receptor cDNAs
- PMID: 3755212
- DOI: 10.1016/0076-6879(86)28113-6
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Molecular cloning of bovine LDL receptor cDNAs
Methods Enzymol.
1986.
Abstract
Using methods described above, a partial cDNA clone for the bovine LDL receptor has been isolated. DNA sequence analysis and Northern blotting experiments are used to confirm the identity of pLDLR-1. Further DNA sequence analysis of pLDLR-1 reveals that the partial cDNA insert encodes 264 amino acids corresponding to the carboxy-terminal 25% of the bovine LDL receptor. Antipeptide antibodies directed against regions of the predicted protein sequence specifically recognize the purified bovine receptor. These findings provide an independent confirmation of the identity of pLDLR-1.
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