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. 1986 Jul 28;203(2):111-5.
doi: 10.1016/0014-5793(86)80724-4.

Cloning of rat brain protein kinase C complementary DNA

Y Ono, T Kurokawa, K Kawahara, O Nishimura, R Marumoto, K Igarashi, Y Sugino, U Kikkawa, K Ogita, Y Nishizuka
Free article

Cloning of rat brain protein kinase C complementary DNA

Y Ono et al. FEBS Lett. .
Free article

Abstract

Four peptides derived from rat brain protein kinase C were partially sequenced. Using synthetic oligonucleotides deduced from the amino acid sequences as probes, a clone of complementary DNA (cDNA) was isolated from a cDNA library prepared from the same tissue. The nucleotide sequence of this cDNA clone revealed the primary structure of the carboxyl-terminal region as having 224 amino acids, with significant sequence homology with cyclic AMP-dependent and cyclic GMP-dependent protein kinases.

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