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. 2023 Jul 10;10(8):ofad357.
doi: 10.1093/ofid/ofad357. eCollection 2023 Aug.

Molecular Characterization of Trypanosoma cruzi Reactivation and Follow-up in a Case Series of People With HIV

Marisa Liliana Fernandez  1   2 , Constanza Lopez Albizu  1 , Diego Nicita  2 , Susana Besuschio  3 , Cinthia Giomi  2 , María Laura Biondi  2 , María Susana Leguizamón  4   5 , Julian Garcia  2 , Marcelo Corti  2 , Alejandro Schijman  3   5 , Juan Miguel Burgos  4   5
Affiliations

Molecular Characterization of Trypanosoma cruzi Reactivation and Follow-up in a Case Series of People With HIV

Marisa Liliana Fernandez et al. Open Forum Infect Dis. .

Abstract

We characterize Trypanosoma cruzi infections from blood and cerebrospinal fluid samples in a case series of people with human immunodeficiency virus and Chagas disease. We identify different infecting T. cruzi populations, highlighting the usefulness of real-time polymerase chain reaction for Chagas disease reactivation diagnosis and evaluation of treatment response.

Keywords: Chagas disease reactivation; Trypanosoma cruzi; people with HIV; qPCR.

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Conflict of interest statement

Potential conflicts of interest. All authors report no potential conflicts of interest.

Figures

Figure 1.
Figure 1.
Minicircle signatures (vkDNA polymerase chain reaction–restriction fragment length polymorphism) of Trypanosoma cruzi populations detected in peripheral blood (PB) and cerebrospinal fluid (CSF) of patients 1 and 2. Subscript numbers indicate days of treatment. Each patient’s parasite population presented its own minicircle signature, with high identity between CSF and PB samples, suggesting infections by similar parasite populations in both tissues. Abbreviations: CSF, cerebrospinal fluid; M, 50-bp molecular marker; PB, peripheral blood.
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