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. 2023:2708:99-106.
doi: 10.1007/978-1-0716-3409-7_10.

Optic Nerve Crush Injury in Rodents to Study Retinal Ganglion Cell Neuroprotection and Regeneration

Zubair Ahmed  1
Affiliations

Optic Nerve Crush Injury in Rodents to Study Retinal Ganglion Cell Neuroprotection and Regeneration

Zubair Ahmed. Methods Mol Biol. 2023.

Abstract

Optic nerve crush injury is a useful model for studying the response of central nervous system neurons (CNS) to injury. A particular focus of this model has been to elucidate therapeutic factors in promoting neuroprotection and axon regeneration after injury. Here we describe a step-by-step protocol in accessing the optic nerve and creating a crush injury. This can be used to create a reproducible model to study the response of retinal ganglion cells (RGC), the main projection neurons of the eye, to injury.

Keywords: Axon regeneration; Central nervous system; Neuroprotection; Optic nerve; Optic nerve crush injury; Retinal ganglion cells.

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