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. 2023 Jul 15;15(7):4533-4543.
eCollection 2023.

Phytochemical, antioxidant, antipyretic and anti-inflammatory activities of aqueous-methanolic leaf extract of Mangifera indica

Qurat Ul Ain  1   2 , Muhammad Omer Iqbal  3   4 , Imran Ahmad Khan  5 , Naheed Bano  6 , Muhammad Naeem  7 , Mohamad Ikhwan Jamaludin  8 , Sutha Devaraj  9
Affiliations

Phytochemical, antioxidant, antipyretic and anti-inflammatory activities of aqueous-methanolic leaf extract of Mangifera indica

Qurat Ul Ain et al. Am J Transl Res. .

Abstract

Objective: Plant-based natural antioxidants have a wide variety of biological activities with significant therapeutic value. Mangifera indica has been used traditionally to treat a variety of ailments in animals and human, but little is defined about its biological or pharmacological effects. Therefore, the objective of the present study was to evaluate phytochemical, antioxidant, antipyretic and anti-inflammatory activities of aqueous-methanolic leaf extract of M. indica.

Methods: To investigate the possible impact of aqueous-methanolic leaf extract of M. indica on oxidative stress, inflammation, and pyrexia, we used a combined in vitro and in vivo series of experiments on laboratory animals.

Results: Results revealed significant antioxidant potential in 2,2-diphenylpicrylhydrazyl (DPPH) and nitric oxide (NO) scavenging assay, while significant but dose dependent antipyretic potential was documented in typhoid-paratyphoid A and B (TAB) vaccine and prostaglandin E (PGE) induced pyrexia models. Significant anti-inflammatory effects were observed in both acute and chronic inflammatory models of arachidonic acid and formalin. Phytochemical screening and high-performance liquid chromatography (HPLC) analysis of M. Indica confirmed the presence of mangiferin, quercetin, and isoquercetin. These phytoconstituents likely play a role in the observed biological activities. Our results show that M. indica has antioxidant, anti-inflammatory, and antipyretic effects, lending credence to its traditional use and advocating for its utilization as a viable contender in treating oxidative stress-associated ailments.

Conclusion: It is concluded that Magnifera indica has various properties in the treatment of various diseases.

Keywords: Inflammation; Mangifera indica; antioxidant; prostaglandins; pyrexia.

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Conflict of interest statement

None.

Figures

Figure 1
Figure 1
HPLC of aqueous-methanolic leaf extract of M. indica indicating the presence of mangiferin (A), quercetin (B) and isoquercetin (C) in comparison to retention time.
Figure 2
Figure 2
Antioxidant potential of aqueous-methanolic leaf extract of M. indica using DPPH assay concerning ascorbic acid. DPPH: 2,2-diphenylpicrylhydrazyl.
Figure 3
Figure 3
Antioxidant potential of aqueous-methanolic leaf extract of M. indica using NO scavenging assay concerning ascorbic acid. NO: Nitric oxide.
Figure 4
Figure 4
Antipyretic potential of aqueous-methanolic leaf extract of M. indica against PGE-induced pyrexia in rabbits. PGE: Prostaglandin E.
Figure 5
Figure 5
Antipyretic potential of aqueous-methanolic leaf extract of M. indica against typhoid-paratyphoid A and B (TAB) vaccine-induced pyrexia in rabbits.
Figure 6
Figure 6
Anti-inflammatory potential of aqueous-methanolic leaf extract of M. indica against arachidonic acid-induced inflammation in rats. * = Significant variation as compared to week zero (P < 0.05). ** = Significant variation as compared to week zero (P < 0.01). NS = Non-significant variation as compared to week zero (P > 0.05).
Figure 7
Figure 7
Anti-inflammatory potential of aqueous-methanolic leaf extract of M. indica against formalin induced inflammation in rats. * = Significant variation as compared to week zero (P < 0.05). ** = Significant variation as compared to week zero (P < 0.01). NS = Non-significant variation as compared to week zero (P > 0.05).
Figure 8
Figure 8
The proposed mechanism of action of active constituents of M. indica (mangiferin, quercetin and isoquercetin) on various parameters.
See this image and copyright information in PMC

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