Biotic and Abiotic Interactions in Freshwater Mesocosms Determine Fate and Toxicity of CuO Nanoparticles

Abstract

Transformation, dissolution, and sorption of copper oxide nanoparticles (CuO-NP) play an important role in freshwater ecosystems. We present the first mesocosm experiment on the fate of CuO-NP and the dynamics of the zooplankton community over a period of 12 months. Increasingly low (0.08-0.28 mg Cu L^-1) and high (0.99-2.99 mg Cu L^-1) concentrations of CuO-NP and CuSO₄ (0.10-0.34 mg Cu L^-1) were tested in a multiple dosing scenario. At the high applied concentration (CuO-NP_H) CuO-NP aggregated and sank onto the sediment layer, where we recovered 63% of Cu applied. For the low concentration (CuO-NP_L) only 41% of applied copper could be recovered in the sediment. In the water column, the percentage of initially applied Cu recovered was on average 3-fold higher for CuO-NP_L than for CuO-NP_H. Zooplankton abundance was substantially compromised in the treatments CuSO₄ (p < 0.001) and CuO-NP_L (p < 0.001). Community analysis indicated that Cladocera were most affected (b_k = -0.49), followed by Nematocera (b_k = -0.32). The abundance of Cladocera over time and of Dixidae in summer was significantly reduced in the treatment CuO-NP_L (p < 0.001; p < 0.05) compared to the Control. Our results indicate a higher potential for negative impacts on the freshwater community when lower concentrations of CuO-NP (<0.1 mg Cu L^-1) enter the ecosystem.

Keywords: Cladocera; community analysis; copper nanoparticle; freshwater mesocosm; long-term field study; nanoparticle fate; population dynamics.