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. 2023 Jul;175(3):362-366.
doi: 10.1007/s10517-023-05868-1. Epub 2023 Aug 10.

The Influence of Permafrost Microorganisms on Monocytes Differentiation In Vitro

S A Petrov  1 , Yu G Sukhovei  1 , L F Kalenova  2 , E G Kostolomova  3 , A M Subbotin  1 , A A Kastornov  1
Affiliations

The Influence of Permafrost Microorganisms on Monocytes Differentiation In Vitro

S A Petrov et al. Bull Exp Biol Med. 2023 Jul.

Abstract

Metabolites of fossil microorganisms of dispersed watered rocks that have passed into a frozen state (Bacillus sp. strains 2/09 and 9/08, Bacillus megaterium 8-75) can modulate the differentiation activity of human peripheral blood monocytes into phenotypically different subpopulations when cultured in vitro for 7 days. This effect is largely determined by the type of metabolites that depends on the temperature of their production: cold (collected after bacterium culturing at 5°C), medium (at 22°C), and warm temperature (at 37°C). All three types of metabolites had a weak negative influence on the level of classical (CD14hiCD16-) monocytes and stimulated the differentiation of intermediate (CD14+CD16+) and non-classical (CD14loCD16+) monocytes. The monocytes differentiation into the subpopulation of intermediate (CD14+CD16+) was stimulated to a greater extent by medium-temperature metabolites of the strain 8/75 and into the subpopulation of non-classical (CD14loCD16+) monocytes by warm metabolites of the strains 8/75 and 2/09 throughout culturing of mononuclear cells (days 1, 3, 7). Bearing in mind the anti-infection activity of intermediate and non-classical monocytes, we can consider strains 8/75 Bacillus megaterium and 2/09 Bacillus sp. promising for their in-depth testing.

Keywords: bacterial metabolites; monocytes differentiation; monocytes differentiation regulatory factors; monocytes subpopulations; permafrost fossil microorganisms.

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