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. 2023 Aug 4;15(15):3297.
doi: 10.3390/polym15153297.

Valorisation of Pineapple Cannery Waste as a Cost Effective Carbon Source for Poly 3-hydroxyabutyrate (P3HB) Production

Waranya Suwannasing  1 , Varavut Tanamool  2 , Pakjirat Singhaboot  3 , Pakawadee Kaewkannetra  4
Affiliations

Valorisation of Pineapple Cannery Waste as a Cost Effective Carbon Source for Poly 3-hydroxyabutyrate (P3HB) Production

Waranya Suwannasing et al. Polymers (Basel). .

Abstract

Pineapple is one of the most important agro-industrial sugar-based fruits in Thailand. In this study, the waste stream from pineapple cannery processing was utilised and evaluated for potential use in the production of a main biopolymer group widely known as polyhydroxyalkanoates (PHAs) through aerobic batch fermentation. Firstly, pineapple cannery waste (PCW) collected from three processing sources, pineapple juice (PAJ), peel and core juice (PCJ), and pulp-washing water (PWW), was used as a carbon source. Secondly, it was characterised and pretreated. Then, batch fermentation was performed by using the optimal condition (200 rpm agitation rate, 37 °C, and fermentation time of 72 h) under two different nutrient conditions in each type of carbon source. The results revealed that PHAs were produced during 24-72 h of fermentation without any interference. The PHAs product obtained was characterised by their properties. Interestingly, GC-MS showed homopolymer of poly 3-hydroxybutyrate (P3HB) group characteristics, such as OH, CH, and C=O; meanwhile, H1 NMR analysis showed signals corresponding to CH3, CH2, and CH, respectively. Remarkably, utilising the PCW showed a high-potential cheap carbon source for the production of PHAs as well as for the treatment of wastewater from the fruit industry.

Keywords: fermentation; pineapple processing waste; polyhydroxyalkanoates (PHAs); polymer.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The schematic diagram of pineapple cannery process.
Figure 2
Figure 2
The profile of PHAs production from pineapple waste by Bacillus sp. SV13 via aerobic batch fermentation with/without adding nutrients.
Figure 3
Figure 3
The profile of PHAs production via a batch fermentation in a 5 L fermentor by using PWW with added nutrients.
Figure 4
Figure 4
The 13C-NMR spectra of PHAs polymer extracted from PAJ, PCJ, and PWW with added nutrients.
See this image and copyright information in PMC

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