Radiation damage to biological macromolecules∗
- PMID: 37573816
- DOI: 10.1016/j.sbi.2023.102662
Radiation damage to biological macromolecules∗
Abstract
In this review, we describe recent research developments into radiation damage effects in macromolecular X-ray crystallography observed at synchrotrons and X-ray free electron lasers. Radiation damage in small molecule X-ray crystallography, small angle X-ray scattering experiments, microelectron diffraction, and single particle cryo-electron microscopy is briefly covered.
Keywords: Disulphide bond breakage; Dose; Electrons; Global damage; Microscopy; Radiation damage; Reduction; Specific structural damage; Synchrotrons; X-ray free electron lasers; X-rays.
Copyright © 2023. Published by Elsevier Ltd.
Conflict of interest statement
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Similar articles
-
Radiation damage to biological samples: still a pertinent issue.J Synchrotron Radiat. 2021 Sep 1;28(Pt 5):1278-1283. doi: 10.1107/S1600577521008845. Epub 2021 Sep 1. J Synchrotron Radiat. 2021. PMID: 34475277 Free PMC article.
-
Radiation Damage in Macromolecular Crystallography.Methods Mol Biol. 2017;1607:467-489. doi: 10.1007/978-1-4939-7000-1_20. Methods Mol Biol. 2017. PMID: 28573586 Review.
-
X-Ray Free-Electron Lasers for the Structure and Dynamics of Macromolecules.Annu Rev Biochem. 2019 Jun 20;88:35-58. doi: 10.1146/annurev-biochem-013118-110744. Epub 2019 Jan 2. Annu Rev Biochem. 2019. PMID: 30601681 Review.
-
Radiation damage to protein specimens from electron beam imaging and diffraction: a mini-review of anti-damage approaches, with special reference to synchrotron X-ray crystallography.J Synchrotron Radiat. 2007 Jan;14(Pt 1):116-27. doi: 10.1107/S0909049506052307. Epub 2006 Dec 15. J Synchrotron Radiat. 2007. PMID: 17211078 Review.
-
Radiation damage in protein crystallography at X-ray free-electron lasers.Acta Crystallogr D Struct Biol. 2019 Feb 1;75(Pt 2):211-218. doi: 10.1107/S2059798319000317. Epub 2019 Jan 28. Acta Crystallogr D Struct Biol. 2019. PMID: 30821709 Free PMC article. Review.
Cited by
-
Full-Field Strain Measurements of the Muscle-Tendon Junction Using X-ray Computed Tomography and Digital Volume Correlation.Bioengineering (Basel). 2024 Feb 6;11(2):162. doi: 10.3390/bioengineering11020162. Bioengineering (Basel). 2024. PMID: 38391648 Free PMC article.
-
Beam Effects in Synchrotron Radiation Operando Characterization of Battery Materials: X-Ray Diffraction and Absorption Study of LiNi0.33Mn0.33Co0.33O2 and LiFePO4 Electrodes.Chem Mater. 2024 May 17;36(11):5596-5610. doi: 10.1021/acs.chemmater.4c00597. eCollection 2024 Jun 11. Chem Mater. 2024. PMID: 38883437 Free PMC article.
-
Sheet-on-sheet fixed target data collection devices for serial crystallography at synchrotron and XFEL sources.J Appl Crystallogr. 2024 Oct 16;57(Pt 6):1725-1732. doi: 10.1107/S1600576724008914. eCollection 2024 Dec 1. J Appl Crystallogr. 2024. PMID: 39628875 Free PMC article.
-
Accounting for electron-beam-induced warping of molecular nanocrystals in MicroED structure determination.IUCrJ. 2025 Mar 1;12(Pt 2):223-238. doi: 10.1107/S2052252524012132. IUCrJ. 2025. PMID: 39927752 Free PMC article.
-
Small but mighty: the power of microcrystals in structural biology.IUCrJ. 2025 May 1;12(Pt 3):262-279. doi: 10.1107/S2052252525001484. IUCrJ. 2025. PMID: 40080159 Free PMC article. Review.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
