Fluorescence-activated sorting of individual cells onto poly-L-lysine-coated slide areas

Abstract

Cells sorted by a fluorescence-activated cell sorter are collected onto small areas of a glass slide. These collection areas have been coated with poly-L-lysine to attach the cells firmly to the glass surface. This simple procedure proved to be suitable to sort single cells and small cell populations with preservation of cytomorphology and viability without modifying the cell sorter. Additional studies on sorted cells may be performed, as shown by peroxidase-anti-peroxidase analysis of cellular antigens and by mRNA in situ hybridization.