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. 2023 Jul 27:14:1200123.
doi: 10.3389/fmicb.2023.1200123. eCollection 2023.

Screening of exopolysaccharide-producing Enterobacter aerogenes NJ1023 and its cadaverine biosynthesis promotion

Yanai Xie  1 , Zhen Ye  1 , Xin Wan  1 , Hua Deng  1 , Weihao Sun  1 , Xun He  1 , Kequan Chen  1
Affiliations

Screening of exopolysaccharide-producing Enterobacter aerogenes NJ1023 and its cadaverine biosynthesis promotion

Yanai Xie et al. Front Microbiol. .

Abstract

Enterobacter aerogenes, the gram-negative bacteria belonging to the family Enterobacteriaceae, lacks the ability to synthesize chemicals. However, in this study, a strain of Enterobacter aerogenes NJ1023 screened from the soil containing petrochemicals was found to be capable of producing extracellular polysaccharides (EPSs). After purification of the polysaccharide, the chemical composition and physicochemical properties of the polysaccharide were analyzed by UV-Vis spectra, FTIR spectroscopy and GC-MS, etc. The results showed that: The molecular weight of the polysaccharide produced by this strain was only 2.7×103 Da, which was lower than that reported in other polysaccharides from the same genus. The polysaccharide produced by E. aerogenes NJ1023 mainly comprised xylose, glucose, galactose, and N-acetylglucosamine with a molar ratio of 0.27: 4.52: 1.74: 0.2, which differed from those reported from the same genus. The results demonstrated that lower incubation temperatures and shaking speeds were more favorable for EPSs synthesis, while higher incubation temperatures and shaking speeds favored cell growth. Additionally, the EPSs produced by E. aerogenes NJ1023 significantly protected the Escherichia coli cells against cadaverine stress. Overall, the discovery of EPSs produced by E. aerogenes increased the diversity of bacterial polysaccharides and broadened the potential applications of this species.

Keywords: EPSs; Enterobacter aerogenes; cadaverine; cytoprotection; extracellular polysaccharides.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Elution curves of crude EPSs on DEAE-cellulose DE-32 column (A) and Sephadex G-25 column (B).
Figure 2
Figure 2
Infrared spectrum of EPSs from Enterobacter aerogenes NJ1023.
Figure 3
Figure 3
Effects of pH on cell growth and polysaccharide production of E. aerogenes NJ1023.
Figure 4
Figure 4
Effects of rotational speed on cell growth and polysaccharide production of E. aerogenes NJ1023.
Figure 5
Figure 5
Effects of culture temperature on cell growth and polysaccharide production of E. aerogenes NJ1023. (A) At a rotational speed of 100 rpm. (B) At a rotational speed of 200 rpm.
Figure 6
Figure 6
Response surface plots of effects of interaction between each factor on DCW and EPSs yield. (A) Effect of pH and temperature interaction on DCW. (B) Effect of rotational speed and temperature interaction on DCW. (C) Effect of pH and temperature interaction on EPSs yields.
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