Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Aug 14;19(8):75.
doi: 10.1007/s11306-023-02041-7.

Light effects on Lasiodiplodia theobromae metabolome cultured in vitro

Alessandre C Crispim  1 , Shirley M A Crispim  2 , Jéssica R Rocha  2 , Jeferson S Ursulino  2 , Roberto R Sobrinho  3 , Viviane A Porto  4 , Edson S Bento  2 , Antônio E G Santana  5 , Luiz C Caetano  2
Affiliations

Light effects on Lasiodiplodia theobromae metabolome cultured in vitro

Alessandre C Crispim et al. Metabolomics. .

Abstract

Introduction: The present work identified and compared intracellular metabolites and metabolic networks in mycelial cultures of Lasiodiplodia theobromae grown under 12 natural light and 24 hours' dark using a 1 H NMR-based metabolomics approach.

Materials and methods: Fungal cultures were grown in potato dextrose media, and metabolites were extracted by sonication with sodium phosphate-buffered saline (pH = 6.0, 10% D2O, 0.1 mM TSP) from mycelium samples collected every week over four weeks.

Results: Multivariate analyses revealed that the light exposure group showed a positive correlation within beta-hydroxybutyrate, acetoacetate, acetone, betaine, choline, glycerol, and phosphocholine. On the other hand, phenyl acetate, leucine, isoleucine, valine, and tyrosine were positively correlated with dark conditions. Light favored the oxidative degradation of valine, leucine, and isoleucine, leading to the accumulation of choline, phosphocholine, betaine, and ketone bodies (ketogenesis). Ketogenesis, gluconeogenesis, and the biosynthesis of choline, phosphocholine, and betaine, were considered discriminatory routes for light conditions. The light-sensing pathways were interlinked with fungal development, as verified by the increased production of mycelia biomass without fruiting bodies and stress signaling, as demonstrated by the increased production of pigments.

Keywords: Botryosphaeriaceae; Metabolomics; Phytopathogen; fungi pigment.

PubMed Disclaimer

References

    1. Calvo, A. M., Wilson, R. A., Bok, J. W., & Keller, N. P. (2002). Relationship between secondary metabolism and fungal development. Microbiology and molecular biology reviews, 66(3), 447–459. https://doi.org/10.1128/MMBR.66.3.447-459.2002 . - DOI - PubMed - PMC
    1. Carlini, E. A., Duarte-Almeida, J. M., Rodrigues, E., & Tabach, R. (2010). Antiulcer effect of the pepper trees Schinus terebinthifolius Raddi (aroeira-da-praia) and myracrodruon urundeuva Allemão, Anacardiaceae (aroeira-do-sertão). Revista Brasileira de Farmacognosia, 20, 140–146. https://doi.org/10.1590/S0102-695X2010000200001 . - DOI
    1. Chen, S. F., Morgan, D., Beede, R. H., & Michailides, T. J. (2013). First report of Lasiodiplodia theobromae associated with stem canker of almond in California. Plant Disease, 97(7), 994–994. https://doi.org/10.1094/PDIS-11-12-1033-PDN . - DOI - PubMed
    1. Cheong, K. K., Strub, C., Montet, D., Durand, N., Alter, P., Meile, J. C., Galindo, S. S., & Fontana, A. (2016). Effect of different light wavelengths on the growth and ochratoxin A production in aspergillus carbonarius and aspergillus westerdijkiae. Fungal Biology, 120(5), 745–751. https://doi.org/10.1016/j.funbio.2016.02.005 . - DOI - PubMed
    1. Darwish, A. M. G. (2019). Fungal mycotoxins and natural antioxidants: Two sides of the same coin and significance in food safety. Microbial Biosystems, 4(1), 1–16. https://doi.org/10.21608/mb.2019.37468 . - DOI

Supplementary concepts

LinkOut - more resources