Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Aug 14;23(1):220.
doi: 10.1186/s12866-023-02964-0.

Mesomycoplasma ovipneumoniae from goats with respiratory infection: pathogenic characteristics, population structure, and genomic features

Chunxia Ma  1   2   3 , Ming Li  4 , Hao Peng  2   3 , Meiyi Lan  2   3 , Li Tao  2   3 , Changting Li  2   3 , Cuilan Wu  2   3 , Huili Bai  2   3 , Yawen Zhong  1 , Shuhong Zhong  2   3 , Ruofu Qin  2   3 , Fengsheng Li  2   3 , Jun Li  5   6 , Jiakang He  7
Affiliations

Mesomycoplasma ovipneumoniae from goats with respiratory infection: pathogenic characteristics, population structure, and genomic features

Chunxia Ma et al. BMC Microbiol. .

Abstract

Background: Mycoplasma ovipneumoniae is a critical pathogen that causes respiratory diseases that threaten Caprini health and cause economic damage. A genome-wide study of M. ovipneumoniae will help understand the pathogenic characteristics of this microorganism.

Results: Toxicological pathology and whole-genome sequencing of nine M. ovipneumoniae strains isolated from goats were performed using an epidemiological survey. These strains exhibited anterior ventral lung consolidation, typical of bronchopneumonia in goats. Average nucleotide identity and phylogenetic analysis based on whole-genome sequences showed that all M. ovipneumoniae strains clustered into two clades, largely in accordance with their geographical origins. The pan-genome of the 23 M. ovipneumoniae strains contained 5,596 genes, including 385 core, 210 soft core, and 5,001 accessory genes. Among these genes, two protein-coding genes were annotated as cilium adhesion and eight as paralog surface adhesins when annotated to VFDB, and no antibiotic resistance-related genes were predicted. Additionally, 23 strains carried glucosidase-related genes (ycjT and group_1595) and glucosidase-related genes (atpD_2), indicating that M. ovipneumoniae possesses a wide range of glycoside hydrolase activities.

Conclusions: The population structure and genomic features identified in this study will facilitate further investigations into the pathogenesis of M. ovipneumoniae and lay the foundation for the development of preventive and therapeutic methods.

Keywords: Mycoplasma ovipneumoniae; Pan-genome; Pathogenesis; Whole-genome sequencing.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Morphology of goat lung tissue. A and B represent cell morphology of goat lung tissue after Mycoplasma ovipneumoniae NN-MO infection. C and D represent morphology of goat lung tissue after M. ovipneumoniae BHF6 infection
Fig. 2
Fig. 2
Hierarchical clustering in two dimensions of pairwise average nucleotide identity (ANI) of 23 Mycoplasma ovipneumoniae strains. Pairwise ANI values are presented as a heatmap
Fig. 3
Fig. 3
Phylogenetic analysis based on the pangenome of 23 Mycoplasma ovipneumoniae strains. Emerald and yellow in outer ring represent the Bayesian analysis of population structure (BAPS) cluster to which each strain is attributed
Fig. 4
Fig. 4
A Pie chart showing the distribution of the core genes, soft-core genes, shell genes and cloud genes present in the pangenome of 23 Mycoplasma ovipneumoniae strains. B Pan-genome and conserved genome. Graph representing the total genes (dotted line) and conserved gene (solid line) of the 23 M. ovipneumoniae strains genomes
Fig. 5
Fig. 5
Distribution of carbohydrate-active enzyme genes in 23 Mycoplasma ovipneumoniae strains genomes
See this image and copyright information in PMC

References

    1. Jay M, Ambroset C, Tricot A, Colin A, Tardy F. Population structure and antimicrobial susceptibility of Mycoplasma ovipneumoniae isolates in France. Vet Microbiol. 2020;248:108828. - PubMed
    1. Mousa WS, Zaghawa AA, Elsify AM, Nayel MA, Ibrahim ZH, Al-Kheraije KA, Elhalafawy HR, El-Shafey D, Anis A, Salama AA. Clinical, histopathological, and molecular characterization of Mycoplasma species in sheep and goats in Egypt. Vet World. 2021;14:2561–2567. - PMC - PubMed
    1. Besser TE, Cassirer EF, Potter KA, Lahmers K, Oaks JL, Shanthalingam S, Srikumaran S, Foreyt WJ. Epizootic pneumonia of bighorn sheep following experimental exposure to Mycoplasma ovipneumoniae. PLoS ONE. 2014;9:e110039. - PMC - PubMed
    1. Manlove K, Branan M, Baker K, Bradway D, Cassirer EF, Marshall KL, Miller RS, Sweeney S, Cross PC, Besser TE. Risk factors and productivity losses associated with Mycoplasma ovipneumoniae infection in United States domestic sheep operations. Prev Vet Med. 2019;168:30–38. - PubMed
    1. Manlove K, Cassirer EF, Cross PC, Plowright RK, Hudson PJ. Disease introduction is associated with a phase transition in bighorn sheep demographics. Ecology. 2016;97:2593–2602. - PMC - PubMed

Publication types

LinkOut - more resources