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. 2023 Jul 29:2023:10.17912/micropub.biology.000934.
doi: 10.17912/micropub.biology.000934. eCollection 2023.

53bp1 mutation enhances brca1 and bard1 embryonic lethality in C. elegans

Sara Hariri  1 , Qianyan Li  1 , JoAnne Engebrecht  1
Affiliations

53bp1 mutation enhances brca1 and bard1 embryonic lethality in C. elegans

Sara Hariri et al. MicroPubl Biol. .

Abstract

In mice, mutation of brca1 results in embryonic lethality, which is partially suppressed by 53bp1 mutation. In contrast, mutation of the C. elegans BRCA1 ortholog, brc-1 , or its binding partner, brd-1 , lead to only mild embryonic lethality. We show that in C. elegans , brc-1 and brd-1 embryonic lethality is enhanced when 53bp1 ortholog, hsr-9 , is also mutated. This is not a consequence of activating polq-1 -dependent microhomology-mediated end joining, as polq-1 mutation does not suppress embryonic lethality of hsr-9 ; brc-1 mutants. Together, these results suggest that BRC-1 - BRD-1 and HSR-9 function in parallel pathways and do not act antagonistically as in mammals.

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Conflict of interest statement

The authors declare that there are no conflicts of interest present.

Figures

Figure 1. <b> Embryonic lethality in different allele combinations of <i>brc-1, brd-1, hsr-9, </i> and <i>polq-1</i> </b>
Figure 1. Embryonic lethality in different allele combinations of brc-1, brd-1, hsr-9, and polq-1
A) Embryonic lethality in wild type (26), brc-1(xoe4) (12), hsr-9(xoe17); brc-1(xoe4) (25), hsr-9(xoe17) (23) , hsr-9(ok759); brc-1(xoe4) (12) , hsr-9(ok759) (12) , hsr-9(xoe17); brc-1(tm1145) (11) , brc-1(tm1145) (11) , hsr-9(xoe17); brd-1(xoe18) (10), and brd-1(xoe18) (12) animals. Number of animals examined are in paratheses. B) Embryonic lethality in hsr-9(xoe17); brc-1(xoe4) (25), hsr-9(xoe17); brc-1(xoe4) polq-1(xoe51) (20) , brc-1(xoe4) polq-1(xoe51) (18) , hsr-9(xoe17); polq-1(xoe45) (10) and polq-1(xoe51) (12). Mean and 95% Confidence Interval shown; *** p < 0.001; ** p < 0.01; ns = not significant by Mann-Whitney.
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