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. 2023 Aug;44(8):801-807.
doi: 10.15537/smj.2023.44.8.20230237.

Prevalence of extended-spectrum β-lactamase and carbapenem-resistant Klebsiella pneumoniae in clinical samples

Amela Dedeić Ljubović  1 , Ðana Granov  1 , Erna Husić  1 , Džemilja Gačanović  1 , Jasmina Halković  1 , Azra Čamdžić Lab Ing  1 , Šejla Kotorić Keser  1 , Svjetlana Loga Zec  1
Affiliations

Prevalence of extended-spectrum β-lactamase and carbapenem-resistant Klebsiella pneumoniae in clinical samples

Amela Dedeić Ljubović et al. Saudi Med J. 2023 Aug.

Abstract

Objectives: To assess the prevalence of these resistant strains in the overall isolates of Klebsiella pneumoniae (K. pneumoniae) in hospital settings.

Methods: This retrospective study was conducted from November 2020 to November 2021. The identification and antibiotic susceptibility testing were performed using standard laboratory methods according to the EUCAST standards. The detection of ESBL and carbapenemase production was performed using phenotypic methods such as E-test, combined-disk test with various inhibitors (ROSCO Diagnostica A/S), chromogenic medium for the detection of ESBL/carbapenemase-producing Enterobacteriaceae (CPE) isolates, and the VITEK 2 Compact system (BioMerieux).

Results: 944 isolates of K. pneumoniae were detected in various clinical specimens. Among these, ESBL-producing strains were detected in 349/944 (37%), whereas carbapenem- resistant strains in 188/944 (20%) of the isolates. The remaining isolates (407/944 [43%]) belonged to the wild type. ESBL isolates were the most common in wound swabs (138 [39.5%]), whereas CRKP isolates in screening samples (110 [58.5%]). The majority of ESBL isolates were detected in surgical departments (105 [30.1%]), whereas CRKP isolates in adult intensive care unit departments (79 [42.%]).

Conclusion: Our results show an increasing frequency of CRKP strains. This presents a significant issue in terms of infection prevention and control in hospital settings.

Keywords: ESBL; Klebsiella pneumoniae; carbapenem resistance; infection prevention and control.

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Figures

Figure 1
Figure 1
- Distribution of extended-spectrum beta-lactamase, Carbapenem-resistant Klebsiella pneumonia (K. pneumoniae), and wild type of K. pneumoniae in clinical samples.
Figure 2
Figure 2
- The overall distribution of isolates according to the specimens and hospital departments. Klebsiella pneumoniae: K. pneumoniae
See this image and copyright information in PMC

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