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. 2023 Aug 16;18(8):e0290145.
doi: 10.1371/journal.pone.0290145. eCollection 2023.

True mineral digestibility in C57Bl/6J mice

Linda F Böswald  1 , Jasmin Wenderlein  2 , Wolfgang Siegert  3 , Reinhard K Straubinger  2 , Ellen Kienzle  1
Affiliations

True mineral digestibility in C57Bl/6J mice

Linda F Böswald et al. PLoS One. .

Abstract

Data on mineral digestibility is key to understand mineral homeostasis and refine the recommendations for the dietary intake of these nutrients. In farm animals and pets, there is plenty of data on mineral digestibility and influencing factors. In laboratory mice, however, there is a lack of information on mineral digestibility under maintenance conditions, although this should be the basis for studies on mineral homeostasis under experimental conditions. The aim of the present study was to analyse data on intake, faecal excretion, and apparent digestibility of calcium, phosphorus, sodium, potassium, and magnesium in C57BL/6J mice fed different maintenance diets with varying voluntary dry matter intake. Lucas-tests were used to quantify true digestibility and describe correlations between dietary intake and excretion/absorption of the nutrients. Calcium, phosphorus, and magnesium showed a linear correlation between intake and faecal excretion (R2: 0.77, 0.93 and 0.91, respectively). Intake and apparently digested amounts of sodium and potassium were correlated linearly (R2: 0.86 and 0.98, respectively). These data show that intake is the major determinant of absorption in the minerals listed above. Faecal calcium and phosphorus excretion were correlated as well (R2 = 0.75).

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Linear correlation between calcium (Ca) intake and faecal excretion (y = 0.67 x + 321.91; R2 = 0.77; Sy.x = 91).
Fig 2
Fig 2. Linear correlation between phosphorus (P) intake and faecal excretion (y = 0.64 x + 53.88; R2 = 0.93; Sy.x = 47).
Fig 3
Fig 3. Linear correlation between faecal calcium (Ca) and phosphorus (P) excretion (y = 0.79 x– 171.07; R2 = 0.75; Sy.x = 87).
Fig 4
Fig 4. Linear correlation between sodium (Na) intake and apparently digested amount (y = 0.92 x– 85.70; R2 = 0.86; Sy.x = 41).
Fig 5
Fig 5. Linear correlation between potassium (K) intake and apparently digested amount (y = 0.76 x + 118.48; R2 = 0.98; Sy.x = 49).
Fig 6
Fig 6. Linear correlation between magnesium (Mg) intake and faecal excretion (y = 0.74 x– 20.69; R2 = 0.91; Sy.x = 34).
See this image and copyright information in PMC

References

    1. Mack J, Alexander L, Morris P, Dobenecker B, Kienzle E. Demonstration of uniformity of calcium absorption in adult dogs and cats. Journal of animal physiology and animal nutrition. 2015;99(5):801–9. doi: 10.1111/jpn.12294 - DOI - PubMed
    1. Böswald L, Dobenecker B, Clauss M, Kienzle E. A comparative meta‐analysis on the relationship of faecal calcium and phosphorus excretion in mammals. Journal of animal physiology and animal nutrition. 2018;102(2):370–9. doi: 10.1111/jpn.12844 - DOI - PubMed
    1. Kienzle E, Burger A. Maintenance requirements of macro-elements in horses. Uebersichten zur Tierernaehrung. 2011;39(2):67–104.
    1. Böswald LF, Klein C, Dobenecker B, Kienzle E. Factorial calculation of calcium and phosphorus requirements of growing dogs. PloS one. 2019;14(8):e0220305. doi: 10.1371/journal.pone.0220305 - DOI - PMC - PubMed
    1. Ausschuss für Bedarfsnormen G. Empfehlungen zur Energie- und Nährstoffversorgung von Pferden. Frankfurt am Main, Germany: DLG-Verlag GmbH; 2014.

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