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. 2023 Aug 16;13(1):13308.
doi: 10.1038/s41598-023-40358-4.

Expression status of circ-SMARCA5, circ-NOL10, circ-LDLRAD3, and circ-RHOT1 in patients with colorectal cancer

Neveen A Hussein  1 , Shehata M El Sewedy  2 , Mohamed M Zakareya  3 , Engy A Youssef  2 , Fawziya A R Ibrahim  2
Affiliations

Expression status of circ-SMARCA5, circ-NOL10, circ-LDLRAD3, and circ-RHOT1 in patients with colorectal cancer

Neveen A Hussein et al. Sci Rep. .

Abstract

Colorectal cancer (CRC) poses a significant burden on both the healthcare systems as well as individuals. The high mortality rate of CRC may be attributed to its metastatic potential, heterogeneity, and delayed diagnosis. CircRNAs are an essential class of regulatory RNAs that play significant roles in cancers. This study aimed to detect the expression status of circ-SMARCA5, circ-NOL10, circ-LDLRAD3, and circ-RHOT1 in patients with CRC. This study included 50 CRC patients, 30 individuals with colorectal diseases (non-cancer), and 20 healthy volunteers. By using real-time PCR, the relative expression of circ-SMARCA5, circ-NOL10, circ-LDLRAD3, and circ-RHOT1 was determined in the collected blood samples. In addition, ECLIA was used to quantify carcinoembryonic antigen (CEA) level. All circRNAs expression and CEA levels were significantly up-regulated in cancer patients (CRC, colon, rectum) as compared to healthy controls, except circ-SMARCA5. Moreover, there was a significant up-regulation of circRNAs in most non-cancer patients (UC, polyp, piles). Insignificant upregulation was observed in circRNAs and CEA when comparing cancer with non-cancer patients. No correlations were found between the studied parameters and most clinicopathological characteristics of cancer and non-cancer patients. Circ-SMARCA5, circ-NOL10, circ-LDLRAD3, and circ-RHOT1 were differentially expressed in patients with CRC as well as in non-cancer patients. Circ-SMARCA5 and circ-NOL10 may act as tumor suppressors, while circ-LDLRAD3 and circ-RHOT1 may be oncogenes. Circ-SMARCA5, circ-NOL10, circ-LDLRAD3, and circ-RHOT1 could be promising markers for the early detection of CRC.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Relative expression of (A) circ-SMARCA5, (B) circ-NOL10, (C) circ-LDLRAD3, (D) circ-RHOT1, (E) CEA in control, UC, polyp, piles, and CRC groups.
Figure 2
Figure 2
Relative expression of (A) circ-SMARCA5, (B) circ-NOL10, (C) circ-LDLRAD3, (D) circ-RHOT1, (E) CEA in control, piles, and rectal cancer groups.
Figure 3
Figure 3
Relative expression of (A) circ-SMARCA5, (B) circ-NOL10, (C) circ-LDLRAD3, (D) circ-RHOT1, (E) CEA in control, UC, polyp, and colon cancer groups.
Figure 4
Figure 4
ROC curves for circ-SMARCA5, circ-NOL10, circ-LDLRAD3, circ-RHOT1 and CEA in (A) CRC from control, (B) UC patients from CRC, (C) polyp patients from CRC, and (D) piles from CRC.
See this image and copyright information in PMC

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