Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Apr 5;11(1):34-37.
doi: 10.1016/j.gendis.2023.03.003. eCollection 2024 Jan.

Helicobacter pylori isolated from gastric juice have higher pathogenic potential than biopsy isolates

Budhadev Baral  1 , Dharmendra Kashyap  1 , Nidhi Varshney  1 , Tarun Prakash Verma  1 , Ajay Kumar Jain  2 , Debi Chatterji  2 , Vinod Kumar  3 , Amit Mishra  4 , Awanish Kumar  5 , Hem Chandra Jha  1
Affiliations

Helicobacter pylori isolated from gastric juice have higher pathogenic potential than biopsy isolates

Budhadev Baral et al. Genes Dis. .
No abstract available

PubMed Disclaimer

Figures

Fig. 1
Figure 1
Gastric juice-derived H. pylori possess a higher carcinogenic ability. (A) The SEM image of the juice (HJ1, HJ10, and HJ14) and biopsy isolates (HB1, HB10, and HB14) was acquired at 4 kV and with a working distance of 4.4 mm. (AI) The representative SEM image of the isolates used in the study. Scale bar = 200 nM. (AII) The graph denoting the length of the isolates in nm. (AIII) The graph showing the width of the isolates in nm. (B,C) The expression level of transcription factors NFκB (65 kDa), β-catenin (85 kDa), and GAPDH (36 kDa) was determined by Western blot. The relative expression level of the molecules was determined using Image J software and represented as a histogram. (BI) Western blot image of NFκB 6, 12, and 24 h post-infection. Relative expression of NFκB 6 h (BII), 12 h (BIII), and 24 h (BIV) post-infection. (CI) Western blot image of β-catenin 6, 12, and 24 h post-infection. Relative expression of β-catenin 6 h (CII), 12 h (CIII), and 24 h (CIV) post-infection. X-axis: samples infected with specific H. pylori isolates; Y-axis: fold change with respect to control. The experiment has been performed in triplicates, and the results are shown as the mean ± standard deviation. Unpaired t-tests were applied to determine the statistical significance. P < 0.05 was considered significant in all the cases. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.0001 for significant up-regulation; #P < 0.05, ##P < 0.01, and ###P < 0.0001 for significant down-regulation. (D) Anoikis assay was performed to assess the ability of AGS cells for anchorage-independent growth. (DI) Representative image of AGS cell clumps (representing resistance to anoikis) 6, 12, and 24 h post-H. pylori infection, followed by 48 h anoikis induction and staining with 0.5% crystal violet. Images were acquired by inverted light microscopy (Leica Ltd.) using a 20× objective lens. (DII) Graphical representation of the clump area (arbitrary unit). The area of the clump was measured using Image J software from the acquired images. 50 clumps were measured for every sample. The experiment was performed in triplicates, and the results are shown as the mean ± standard deviation. Unpaired t-tests were applied to determine the statistical significance. P < 0.05 was considered significant in all the cases. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.0001. (E) A model illustrating the difference in growth, morphology, and pathogenic ability of H. pylori isolates from gastric biopsy and juice. Although both juice and biopsy isolates accelerate the occurrence of cancerous properties, the juice isolates possess a higher carcinogenic ability. There is increased β catenin expression and nuclear localization at the later time points which may reduce the expression of NFκB, which is up-regulated at the earlier time points. The inflammatory genes also get highly up-regulated in juice isolate infected samples. Our study showed the difference in the juice and biopsy H. pylori isolates from the same subjects. This is a preliminary report and further study is warranted in this aspect.
figs1
figs1
figs2
figs2
figs3
figs3
See this image and copyright information in PMC

References

    1. Conteduca V., Sansonno D., Lauletta G., et al. H. pylori infection and gastric cancer: state of the art. Int J Oncol. 2013;42(1):5–18. - PubMed
    1. Kashyap D., Baral B., Jakhmola S., et al. Helicobacter pylori and Epstein-Barr virus coinfection stimulates aggressiveness in gastric cancer through the regulation of gankyrin. mSphere. 2021;6(5) - PMC - PubMed
    1. Kashyap D., Baral B., Verma T.P., et al. Oral rinses in growth inhibition and treatment of Helicobacter pylori infection. BMC Microbiol. 2020;20(1):45. - PMC - PubMed
    1. Cao L., Zhu S., Lu H., et al. Helicobacter pylori-induced RASAL2 through activation of nuclear factor-κB promotes gastric tumorigenesis via β-catenin signaling axis. Gastroenterology. 2022;162(6):1716–1731. - PMC - PubMed
    1. Deng J., Miller S.A., Wang H.Y., et al. Beta-catenin interacts with and inhibits NF-kappa B in human colon and breast cancer. Cancer Cell. 2002;2(4):323–334. - PubMed

LinkOut - more resources