Enzyme assay of sialic acid 9-phosphate synthase (SPS)
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Enzyme assay of sialic acid 9-phosphate synthase (SPS)
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References
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- Nakata D, Close BE, Colley KJ, Matsuda T, Kitajima K. Molecular cloning and expression of the mouse N-acetylneuraminic acid 9-phosphate synthase which does not have deaminoneuraminic acid (KDN) 9-phosphate synthase activity. Biochem Biophys Res Commun. 2000;273:642–8. doi: 10.1006/bbrc.2000.2983. - DOI - PubMed
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- Kim K, Lawrence SM, Park J, Pitts L, Vann WF, Betenbaugh MJ, Palter KB. Expression of a functional Drosophila melanogaster N-acetylneuraminic acid (Neu5Ac) phosphate synthase gene: evidence for endogenous sialic acid biosynthetic ability in insects. Glycobiology. 2002;12:73–83. doi: 10.1093/glycob/12.2.73. - DOI - PubMed
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- Willems AP, Sun L, Schulz MA, Tian W, Ashikov A, van Scherpenzeel M, Hermans E, Clausen H, Yang Z, Lefeber DJ. Activity of N-acylneuraminate-9-phosphatase (NANP) is not essential for de novo sialic acid biosynthesis. Biochim Biophys Acta Gen Subj. 2019;1863:1471–9. doi: 10.1016/j.bbagen.2019.05.011. - DOI - PubMed
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