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. 2024 Feb;29(1):100103.
doi: 10.1016/j.slast.2023.08.003. Epub 2023 Aug 16.

Automated glycan-bead coupling for high throughput, highly reproducible anti-glycan antibody analysis

Antonia Katharina Hefermehl  1 , Sanne Maria Mathias Hensen  2 , Carina Versantvoort  3 , Andrée Rothermel  3 , Uğur Şahin  4
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Free article

Automated glycan-bead coupling for high throughput, highly reproducible anti-glycan antibody analysis

Antonia Katharina Hefermehl et al. SLAS Technol. 2024 Feb.
Free article

Abstract

Automation of diagnostic assays generally aims to increase reproducibility and throughput while decreasing human errors and hands-on time. Here, we introduce a protocol for the automated chemical conjugation of glycans to color-coded magnetic beads using the KingFisher Flex magnetic particle processor. The resulting glycan-coupled magnetic beads allow the detection of anti-glycan antibodies of different isotypes from various species. By generating anti-glycan antibody profiles, monoclonal antibodies can be screened for their specificity and cross-reactivity, while anti-glycan antibody profiles from different human body fluids can aid in predicting response to treatment or outcome of disease. This efficient, scalable protocol can also be adapted to attach proteins and other biomolecules to beads, making it useful for a wider range of applications that require bead-based laboratory methods.

Keywords: Anti-glycan antibodies; Laboratory automation; Luminex.

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Conflict of interest statement

Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Ugur Sahin reports a relationship with BioNTech SE that includes: board membership, employment, and equity or stocks. Sanne Maria Mathias Hensen reports a relationship with BioNTech Diagnostics GmbH that includes: employment and equity or stocks.

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