Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Randomized Controlled Trial
. 2023 Sep;63(9):1649-1660.
doi: 10.1111/trf.17503. Epub 2023 Aug 19.

A randomized cross-over study of cryopreserved platelets in prophylactic transfusions of thrombocytopenic patients

Ai Leen Ang  1   2 , Linda Seo Hwee Gan  3 , Tertius Tansloan Tuy  1 , Chieh Hwee Ang  1 , Chuen Wen Tan  1 , Hwee Huang Tan  2 , Pei Huey Shu  2 , Qingxiang Zhang  3 , Yang Cao  4 , Rajesh Babu Moorakonda  4 , Yogesh Pokharkar  4 , Jia Lu  3
Affiliations
Randomized Controlled Trial

A randomized cross-over study of cryopreserved platelets in prophylactic transfusions of thrombocytopenic patients

Ai Leen Ang et al. Transfusion. 2023 Sep.

Abstract

Background: The short shelf-life of liquid-stored platelets (LP) at 20-24°C poses shortage and wastage challenges. Cryopreserved platelets have significantly extended shelf-life, and were safe and efficacious for therapeutic transfusions of bleeding patients in the Afghanistan conflict and phase 2 randomized studies. Although hematology patients account for half of platelets demand, there is no randomized study on prophylactic cryopreserved platelet transfusions in them.

Methods: We performed a phase 1b/2a randomized cross-over study comparing the safety and efficacy of cryopreserved buffy coat-derived pooled platelets (CP) to LP in the prophylactic transfusions of thrombocytopenic hematology patients.

Results: A total of 18 adults were randomly assigned 1:1 to CP and LP for their first thrombocytopenic period (TP) of up to 28-days. A total of 14 crossed over to the other platelet-arm for the second TP. Overall, 17 subjects received 51 CP and 15 received 52 LP. CP-arm had more treatment emergent adverse event (29.4% vs. 13.3% of subjects, 9.8% vs. 3.8% of transfusions) than LP-arm but all were mild. No thromboembolism was observed. Both arms had similar bleeding rates (23.5% vs. 26.7% of subjects) which were all mild. Subjects in CP-arm had lower average corrected count increments than LP-arm (mean [SD] 5.6 [4.20] vs. 22.6 [9.68] ×109 /L at 1-4 h, p < .001; 5.3 [4.84] vs. 18.2 [9.52] ×109 /L at 18-30 h, p < .001). All TEG parameters at 1-4 h and maximum amplitude (MA) at 18-30 h improved from baseline post-CP transfusion (p < .05) though improvements in K-time and MA were lower than LP (p < .05).

Discussion: During shortages, CP may supplement LP in prophylactic transfusions of thrombocytopenic patients.

Keywords: bone; cryopreserved; platelets; prophylactic; subjects; thrombocytopenic.

PubMed Disclaimer

References

REFERENCES

    1. Stubbs JR, Shaz BH, Vasallo RR, et al. Expanding the platelet inventory to mitigate the impact of severe shortages. Hematology Am Soc Hematol Educ Program. 2022;424-429:424-429.
    1. Milford EM, Reade MC. Comprehensive review of platelet storage methods for use in the treatment of active hemorrhage. Transfusion. 2016;56:S140-S180.
    1. Noorman F, van Dongen TT, Plat MJ, et al. Transfusion: −80°C frozen blood products are safe and effective in military casualty care. PloS One. 2016;11:e0168401. https://doi.org/10.1371/journal.pone.0168401
    1. Khuri SF, Healey N, MacGregor H, Barnard MR, Szymanski IO, Birjiniuk V, et al. Comparison of the effects of transfusions of cryopreserved and liquid-preserved platelets on hemostasis and blood loss after cardiopulmonary bypass. J Thorac Cardiovasc Surg. 1999;117:172-183.
    1. Reade MC, Marks DC, Bellomo R, Deans R, Faulke DJ, Fraser JF, et al. A randomized, controlled pilot clinical trial of cryopreserved platelets for perioperative surgical bleeding: the CLIP-I trial. Transfusion. 2019;59:2794-2804.

Publication types

LinkOut - more resources