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. 2023 Aug 19;190(9):357.
doi: 10.1007/s00604-023-05926-5.

Construction of chiral capillary electrochromatography microsystems based on Aspergillus sp. CM96

Tingting Hong  1 , Xing Liu  1 , Yibing Ji  2 , Songwen Tan  3   4 , Zhiqiang Cai  5   6
Affiliations

Construction of chiral capillary electrochromatography microsystems based on Aspergillus sp. CM96

Tingting Hong et al. Mikrochim Acta. .

Abstract

Novel chiral capillary electrochromatography (CEC) microsystems were constructed based on Aspergillus sp. CM96. As a newly discovered intrinsic characteristic of the cell, cell chirality occupies an essential position in life evolution. Aspergillus sp. CM96 spore (CM96s) was chosen as a proof of concept to develop chiral capillary columns. Interestingly, various types of amino acid (AA) enantiomers were baseline separated under the optimized conditions. Furthermore, the time-dependent chiral interactions between AAs and CM96s were explored in a wider space. Pectinases generated from Aspergillus sp. CM96 fermentation were immobilized onto graphene oxide-functionalized capillary silica monoliths for separating AA enantiomers. Molecular docking simulations were performed to explore chiral separation mechanisms of pectinase for AA enantiomers. These results indicated that Aspergillus sp. CM96-based CEC microsystems have a significant advantage for chiral separation.

Keywords: Amino acid; Capillary electrophoresis; Chirality; Graphene oxide; Pectinase; Silica monoliths.

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