Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Nov;240(4):1484-1496.
doi: 10.1111/nph.19207. Epub 2023 Aug 20.

Wound-response jasmonate dynamics in the primary vasculature

Hugo Morin  1   2 , Aurore Chételat  3 , Stéphanie Stolz  3 , Laurence Marcourt  1   2 , Gaëtan Glauser  4 , Jean-Luc Wolfender  1   2 , Edward E Farmer  3
Affiliations
Free article

Wound-response jasmonate dynamics in the primary vasculature

Hugo Morin et al. New Phytol. 2023 Nov.
Free article

Abstract

The links between wound-response electrical signalling and the activation of jasmonate synthesis are unknown. We investigated damage-response remodelling of jasmonate precursor pools in the Arabidopsis thaliana leaf vasculature. Galactolipids and jasmonate precursors in primary veins from undamaged and wounded plants were analysed using MS-based metabolomics and NMR. In parallel, DAD1-LIKE LIPASEs (DALLs), which control the levels of jasmonate precursors in veins, were identified. A novel galactolipid containing the jasmonate precursor 12-oxo-phytodienoic acid (OPDA) was identified in veins: sn-2-O-(cis-12-oxo-phytodienoyl)-sn-3-O-(β-galactopyranosyl) glyceride (sn-2-OPDA-MGMG). Lower levels of sn-1-OPDA-MGMG were also detected. Vascular OPDA-MGMGs, sn-2-18:3-MGMG and free OPDA pools were reduced rapidly in response to damage-activated electrical signals. Reduced function dall2 mutants failed to build resting vascular sn-2-OPDA-MGMG and OPDA pools and, upon wounding, dall2 produced less jasmonoyl-isoleucine (JA-Ile) than the wild-type. DALL3 acted to suppress excess JA-Ile production after wounding, whereas dall2 dall3 double mutants strongly reduce jasmonate signalling in leaves distal to wounds. LOX6 and DALL2 function to produce OPDA and the non-bilayer-forming lipid sn-2-OPDA-MGMG in the primary vasculature. Membrane depolarizations trigger rapid depletion of these molecules. We suggest that electrical signal-dependent lipid phase changes help to initiate vascular jasmonate synthesis in wounded leaves.

Keywords: DALL; OPDA; galactolipid; lipase; slow-wave potential; vein.

PubMed Disclaimer

References

    1. Acosta IF, Gasperini D, Chételat A, Stolz S, Santuari L, Farmer EE. 2013. Role of NINJA in root jasmonate signaling. Proceedings of the National Academy of Sciences, USA 110: 15473-15478.
    1. Buseman CM, Tamura P, Sparks AA, Baughman EJ, Maatta S, Zhao J, Roth MR, Esch SW, Shah J, Williams TD et al. 2006. Wounding stimulates the accumulation of glycerolipids containing oxophytodienoic acid and dinor-oxophytodienoic acid in Arabidopsis leaves. Plant Physiology 142: 28-39.
    1. Caldelari D, Wang G, Farmer EE, Dong X. 2011. Arabidopsis lox3 lox4 double mutants are male sterile and defective in global proliferative arrest. Plant Molecular Biology 75: 25-33.
    1. Challal S, Queiroz E, Debrus B, Kloeti W, Guillarme D, Gupta M, Wolfender J-L. 2015. Rational and efficient preparative isolation of natural products by MPLC-UV-ELSD based on HPLC to MPLC gradient transfer. Planta Medica 81: 1636-1643.
    1. Chauvin A, Caldelari D, Wolfender J, Farmer EE. 2013. Four 13-lipoxygenases contribute to rapid jasmonate synthesis in wounded Arabidopsis thaliana leaves: a role for lipoxygenase 6 in responses to long-distance wound signals. New Phytologist 197: 566-575.

Publication types

LinkOut - more resources