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. 2012 Jul;43(1-2):289-297.
doi: 10.1007/s00723-012-0348-3. Epub 2012 May 20.

Measurement of Arterial Input Function in Hyperpolarized 13C Studies

Małgorzata Marjańska  1 , Thomas Z Teisseyre  2   3 , Nicholas W Halpern-Manners  2   4 , Yi Zhang  1 , Isabelle Iltis  1 , Vikram Bajaj  2   4 , Kamil Ugurbil  1 , Alexander Pines  2   4 , Pierre-Gilles Henry  1
Affiliations

Measurement of Arterial Input Function in Hyperpolarized 13C Studies

Małgorzata Marjańska et al. Appl Magn Reson. 2012 Jul.

Abstract

Recently, hyperpolarized substrates generated through dynamic nuclear polarization have been introduced to study in vivo metabolism. Injection of hyperpolarized [1-13C]pyruvate, the most widely used substrate, allows detection of time courses of [1-13C]pyruvate and its metabolic products, such as [1-13C]lactate and 13C-bicarbonate, in various organs. However, quantitative metabolic modeling of in vivo data to measure specific metabolic rates remains challenging without measuring the input function. In this study, we demonstrate that the input function of [1-13C]pyruvate can be measured in vivo in the rat carotid artery using an implantable coil.

Keywords: DNP; blood; pyruvate.

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Figures

Figure 1.
Figure 1.
Implantable radiofrequency coil. (A) Schematic of the coil. The top layer is outlined in black and the bottom layer is outlined in gray. (B) Picture of fully assembled coil with twisted pair of leads epoxied for support. Leads were then connected to a resonant circuit.
Figure 2.
Figure 2.
The representative spectra obtained with the radiofrequency coil implanted around the CCA. (A) Spectrum obtained 7.8 s after the beginning of injection with the maximum pyruvate and pyruvate hydrate signals observed. (B) Spectrum obtained 22.8 s after the beginning of injection with the maximum lactate signal observed. Repetition time (TR) = 0.6 s, pulse width (pw) = 120 μs, line-broadening = 10 Hz.
Figure 3.
Figure 3.
Three (black, gray, and open squares) time courses of (A) pyruvate and (B) pyruvate hydrate signals obtained with the radiofrequency coil implanted around CCA after three injections of hyperpolarized [1-13C]pyruvate and [1-13C]pyruvate hydrate mixtures. The pyruvate time courses are shown scaled to the highest integral value in each time course. The pyruvate hydrate time courses are shown scaled to the average of the ratio of pyruvate hydrate to pyruvate of all time courses. The inset shows the change in concentration of pyruvate in the blood measured in bench experiment under identical conditions as in in vivo experiments with implantable neck coil. TR = 0.6 s, pw = 120 μs
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