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. 2023:2682:33-58.
doi: 10.1007/978-1-0716-3283-3_3.

Recombinant Soluble Henipavirus Glycoprotein Preparation

Lianying Yan  1 , Spencer L Sterling  1 , Deborah L Fusco  1 , Yee-Peng Chan  1 , Kai Xu  2   3 , Eric D Laing  1 , Christopher C Broder  4
Affiliations

Recombinant Soluble Henipavirus Glycoprotein Preparation

Lianying Yan et al. Methods Mol Biol. 2023.

Abstract

Henipaviruses possess two envelope glycoproteins, the attachment (G) and the fusion (F) proteins that mediate cellular entry and are the major targets of virus-neutralizing antibody responses. Recombinant expression technologies have been used to produce soluble G and F proteins (sG and sF) that retain native-like oligomeric conformations and epitopes, which are advantageous for the development and characterization of vaccines and antiviral antibody therapeutics. In addition to Hendra virus and Nipah virus tetrameric sG and trimeric sF production, we also describe the expression and purification of Cedar virus tetrameric sG and Ghana virus trimeric sF glycoproteins. These henipavirus glycoproteins were also used as immunizing antigens to generate monoclonal antibodies, and binding was demonstrated with a pan-henipavirus multiplex microsphere immunoassay.

Keywords: Cedar virus; Cross-linking; Expression; F glycoprotein; G glycoprotein; Ghana virus; Hendra virus; Henipaviruses; Multiplex microsphere immunoassay; Mòjiāng virus; Nipah virus; Purification.

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References

    1. Wang LF, Yu M, Hansson E, Pritchard LI, Shiell B, Michalski WP, Eaton BT (2000) The exceptionally large genome of Hendra virus: support for creation of a new genus within the family Paramyxoviridae. J Virol 74(21):9972–9979 - DOI - PubMed - PMC
    1. Selvey LA, Wells RM, McCormack JG, Ansford AJ, Murray K, Rogers RJ, Lavercombe PS, Selleck P, Sheridan JW (1995) Infection of humans and horses by a newly described morbillivirus. Med J Aust 162(12):642–645 - DOI - PubMed
    1. Centers for Disease C, Prevention (1999) Outbreak of Hendra-like virus – Malaysia and Singapore, 1998–1999. MMWR Morb Mortal Wkly Rep 48(13):265–269
    1. Mohd Nor MN, Gan CH, Ong BL (2000) Nipah virus infection of pigs in peninsular Malaysia. Rev Sci Tech 19(1):160–165 - DOI - PubMed
    1. Chua KB, Goh KJ, Wong KT, Kamarulzaman A, Tan PS, Ksiazek TG, Zaki SR, Paul G, Lam SK, Tan CT (1999) Fatal encephalitis due to Nipah virus among pig-farmers in Malaysia. Lancet 354(9186):1257–1259. https://doi.org/10.1016/S0140-6736(99)04299-3 - DOI - PubMed

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