Ovarian follicular development during early pregnancy in cattle

Abstract

On the day of oestrus (Day 0), 23 cows were assigned randomly to 4 groups in a 2 X 2 factorial experiment. Main effects were cow status [pregnant (P) versus non-pregnant (NP; not mated)] and day (Day 17 versus Day 34) when cows were slaughtered and ovaries collected; i.e. Groups NP-17 (N = 6), NP-34 (N = 5), P-17 (N = 6) and P-34 (N = 6). To mimic CL maintenance associated with presence of a conceptus in Group P-34, cows assigned to Group NP-34 were hysterectomized between Days 4 and 6 after oestrus. Pregnancy in Groups P-17 and P-34 (recovery of conceptus) and CL maintenance between day of surgery and Day 34 in Group NP-34 were confirmed. In Group NP-17, all cows except one were slaughtered before luteolysis. CL-bearing ovaries were serially sectioned (10 microns) and follicles (greater than 0.15 mm) were counted and measured using routine histological techniques. For each size class of follicles, the percentages of follicles were similar (P greater than 0.1) on Days 17 and 34. The percentage of non-atretic follicles (less than 4 pycnotic bodies) was lower (P less than 0.002) in pregnant than non-pregnant groups in the smallest class of antral follicles (0.16-0.28 mm) but was higher (P less than 0.03) in larger classes (0.68-3.67 mm) for pregnant groups. Percentages of atretic follicles were 3.7 and 4.9 (P greater than 0.1) for follicles of 0.16-0.67 mm, 91.1 and 78.9 for follicles of 0.68-3.67 mm (P less than 0.04) and 5.1 and 16.1 (P less than 0.05) for follicles of 3.67-78.56 mm in pregnant and non-pregnant groups respectively.(ABSTRACT TRUNCATED AT 250 WORDS)