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. 2023 Jul;13(7):873-878.
doi: 10.5455/OVJ.2023.v13.i7.9. Epub 2023 Jul 31.

Sperm abnormality and infertility in male mice treated with the recommended dose of dimethoate and its double

Samira Musa Sasi  1 , Nagia Musa Alghoul  2 , Nuri Awayn  3 , Ahmed Elghoul  4 , Ragil Angga Prastiya  5
Affiliations

Sperm abnormality and infertility in male mice treated with the recommended dose of dimethoate and its double

Samira Musa Sasi et al. Open Vet J. 2023 Jul.

Abstract

Background: Dimethoate (DM) is one of the most important organophosphate insecticides used for controlling many pests which affect vegetables, fruits, and agricultural crops, its persistence in soils and crops could cause a health hazard to humans as well as other non-target organisms.

Aim: This study was conducted to evaluate the effect of the recommended dose and its double of DM on sex hormones, sperm morphology, and fertility of adult male mice.

Methods: Twenty-seven Swiss albino adult male mice were divided into three groups of nine animals each: control group received distilled water only, while other groups received DM orally at doses (0.1 and 0.2 ml DM/100 ml distilled water) for 20 days, at the end of the treatment, six mice from each group were sacrificed. The sperm morphology was evaluated and sex hormones were measured. Three mice from each group were allowed to mate with untreated females (1:2).

Result: The results revealed a decrease in luteinizing hormone levels in mice treated with (0.2 ml DM/100 ml distilled water) compared with the control group while the levels of follicle-stimulating hormone and testosterone did not record any significant differences. Also, the results demonstrated a significant increase in abnormal sperm morphology such as head and tail. The fertility was reduced and the average number of dead embryos increased while the average number of live embryos decreased.

Conclusion: This current study confirmed that DM has detrimental effects on sperm morphology, fertility, and the embryos; therefore, more efforts should be exerted to protect ourselves and our environment from the harmful effects of this pesticide.

Keywords: Dimethoate; Male mice; Reproduction; Sex hormones.

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Conflict of interest statement

All authors declare that there is no conflict of interest.

Figures

Fig. 1.
Fig. 1.. Normal sperm with a hook (400×).
Fig. 2.
Fig. 2.. Microscopic observation of sperm tail abnormalities (400×). (A) Irregular tail; (B) tailless head; and (C) coiled tail.
Fig. 3.
Fig. 3.. Microscope observation of sperm head abnormalities (400×). (A) Headless tail and (B) amorphous head.
See this image and copyright information in PMC

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