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. 2023 Jul;13(7):839-845.
doi: 10.5455/OVJ.2023.v13.i7.5. Epub 2023 Jul 31.

Effect of humic acid and probiotics on immunity of broiler chickens

Fouziyah Ahfeethah  1 , Altayeb Elazomi  2 , Abdulwahab Kammon  3   4
Affiliations

Effect of humic acid and probiotics on immunity of broiler chickens

Fouziyah Ahfeethah et al. Open Vet J. 2023 Jul.

Abstract

Background: The immune system in chickens has a fundamental role in controlling many diseases based on vaccination, thus enhancement of the immune system response is a priority.

Aim: The aim of this experiment was to study the effect of probiotics and humic acid on immunity of broiler chickens.

Methods: Day-old 300 Ross broiler chicks were segregated into 5 groups of 60 chicks per group. Group C was considered as a control. Groups T1, T2, T3, and T4 were given probiotics, antibiotics, humic acid for the first 7 days and humic acid for 42 days, respectively. Samples were collected on days 27 and 42 to assess the humoral immunity, cellular immunity, lymphoid organs weight, and differential leucocyte count (DLC).

Results: The results showed a significant increase (p < 0.05) in antibodies titer against Newcastle disease virus in chickens given humic acid (T4) daily for 42 days as compared to the control. There was also a significant increase in antibodies titer in the T1 group given probiotic for the first week lasting up to day 27 as compared to the control. The skin thickness of T4 group showed a significant increase as compared to T1 and T2 groups after 24 hours of DNCB challenge. After 48 hours, the thickness was still significantly higher in the T4 group as compared to other groups except for the control. There were no significant differences in Bursa of Fabricius/Body weight (%) between the groups. Spleen/Body weight (%) was significantly higher in the control group and T1 than the other groups on day 42. The DLC remains normal in all groups.

Conclusion: It is concluded that the humic acid has a stimulant and strengthening effect on the humoral and cellular immune system when given daily to broiler chickens. Moreover, the use of humic acid and probiotics with good hygiene in the first week of age may alternate the use of antibiotics which could be toxic and raise bacterial resistance.

Keywords: Broiler chickens; Cellular immunity; Humic acid; Humoral immunity; Probiotics.

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Conflict of interest statement

The authors declared that there is no conflict of interest.

References

    1. Arif M, Alagawany M, Abd El-Hack M.E, Saeed M, Arain M.A, Elnesr S.S. Humic acid as a feed additive in poultry diets: a review. Iran J. Vet. Res. 2019;20(3):167–172. - PMC - PubMed
    1. Awad W.A, Böhm J, Razzazi-Fazeli E, Ghareeb K, Zentek J. Effect of addition of a probiotic microorganism to broiler diets contaminated with deoxynivalenol on performance and histological alterations of intestinal villi of broiler chickens. Poult. Sci. 2006;85(6):974–979. - PubMed
    1. Aziz Mousavi S.M.A, Mahmoodzadeh Hosseini H, Mirhosseini S.A. A review of dietary probiotics in poultry. J. Appl. Biotechnol. Rep. 2018;5(2):48–54.
    1. Brisbin J.T, Zhou H, Gong J, Sabour P, Akbari M.R, Haghighi H.R, Yu H, Clarke A, Sarson A.J, Sharif S. Gene expression profiling of chicken lymphoid cells after treatment with Lactobacillus acidophilus cellular components. Dev. Comp. Immunol. 2008;32(5):563–574. - PubMed
    1. Ceylan N, Ciftci I, Ilhan Z. The effects of some alternative feed additives for antibiotic growth promoters on the performance and gut microflora of broiler chicks. Turk. J. Vet. Anim. Sci. 2003;27:727–733.

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