Tau-Marin Mucoadhesive Gel for Prevention and Treatment of Gum Diseases

Abstract

An innovative and stable probiotic-containing mucoadhesive gel (AL0020), integrated with botanical extracts, has been developed to rebalance the dysbiosis associated with periodontal diseases. Tau-Marin gel, prepared with anhydrous ingredients to prevent the replication of bacteria and ensure good stability over time, was tested against some pathogenic bacteria, belonging to the so-called "red complex", recognized as the most important pathogens in plaque specimens, adherent to the epithelial lining of periodontal pockets. This lipogel was tested in vitro, in a physiological solution (PS) and in a simulated saliva (SS), for up to 8 h, to monitor its ability to release probiotics over time. Probiotics were enumerated through two different techniques, Lacto-Counter Assay (LCA) and Colony Forming Unit (CFU). A detailed physico-chemical profile of AL0020 and its in vitro efficacy in protecting activity against pathogenic bacteria as well as soothing or irritative effect on gingival epithelium were reported. Moreover, a clinical-dermatological trial on 20 volunteers using the product once a day for 30 days was also performed, where the efficacy of the gel in the control of gum disorders was observed.

Keywords: Tau-Marin; mucoadhesive gel; oral formulation; periodontal diseases; periodontitis; probiotics; slow delivery.

Figure 1

Viability and IL-1a expression of Tau-Marin gel versus negative and positive controls on a gingival epithelium model, used as protective agent against an irritative agent (0.5% SDS).

Figure 2

Viability and IL-1a expression of Tau-Marin gel versus a negative and positive controls on a gingival epithelium model, used as a soothing agent in counteracting the effects of an irritant agent (0.5% SDS).

Figure 3

Irritative effect of Tau-Marin gel versus negative and positive control, evaluated on a human oral mucosa model after an application lasting four hours.