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Review
. 2023 Jul 26;12(8):978.
doi: 10.3390/pathogens12080978.

The Role of Next-Generation Sequencing (NGS) in the Management of Tuberculosis: Practical Review for Implementation in Routine

Marion Beviere  1 , Sophie Reissier  1   2 , Malo Penven  1   2 , Loren Dejoies  1   2 , François Guerin  1   2   3 , Vincent Cattoir  1   2   3 , Caroline Piau  1
Affiliations
Review

The Role of Next-Generation Sequencing (NGS) in the Management of Tuberculosis: Practical Review for Implementation in Routine

Marion Beviere et al. Pathogens. .

Abstract

Next-generation sequencing (NGS) has modernized the field of tuberculosis (TB) research by enabling high-throughput sequencing of the entire genome of Mycobacterium tuberculosis (MTB), which is the causative agent of TB. NGS has provided insights into the genetic diversity of MTB, which are crucial for understanding the evolution and transmission of the disease, and it has facilitated the identification of drug-resistant strains, enabling rapid and accurate tailoring of treatment. However, the high cost and the technical complexities of NGS currently limit its widespread use in clinical settings. International recommendations are thus necessary to facilitate the interpretation of polymorphisms, and an experimental approach is still necessary to correlate them to phenotypic data. This review aims to present a comparative, step-by-step, and up-to-date review of the techniques available for the implementation of this approach in routine laboratory workflow. Ongoing research on NGS for TB holds promise for improving our understanding of the disease and for developing more efficacious treatments.

Keywords: antibiotic resistance; next-generation sequencing; tuberculosis.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Advantages and limits of molecular techniques for detection of resistance mutations in MTB.
Figure 2
Figure 2
NGS workflow from sample to sequences analysis; stars symbolize critical steps of the process.
See this image and copyright information in PMC

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