Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2023 Nov;87(5):445-447.
doi: 10.1016/j.jinf.2023.08.008. Epub 2023 Aug 23.

Missing subunits of Mpox replication complex: Possible impact on structural organization

Saathvik R Kannan  1 , Athreya S Reddy  2 , Dana M Burris  1 , Siddappa N Byrareddy  3 , Kamal Singh  4
Affiliations
Comment

Missing subunits of Mpox replication complex: Possible impact on structural organization

Saathvik R Kannan et al. J Infect. 2023 Nov.
No abstract available

PubMed Disclaimer

Conflict of interest statement

Declaration of Competing Interest KS is a consultant for Sanctum Therapeutics Corporation, Sunnyvale, CA, USA.

Figures

Figure 1.
Figure 1.. Modeled complete structure of primase-NTPase and the topological position of distinct RC components.
Panel A shows the structure of complete primase-NTPase (OPG117) based on PDB entry 8APM (residues 321-784). The primase domain of OPG117 (1-321) was modeled using AlphaFold through ColabFold. The obtained protein model’s overlap region (321-400) was superposed onto the existing helicase structure region (321-400). Panel B shows a proposed RC consisting of four viral proteins. The structure of DNA polymerase is from the Protein Databank entry 8HG1 {Peng, 2023 #93}. The palm subdomain of the DNA polymerase subunit in 8HG1 was superposed onto the previously modeled structure of MPXV {Kannan, 2022 #94}. The template (cyan) and primer (orange) were modeled using the DNA-bound structure HSV pol (PDB entry 7LUF) and S. cerevisiae replicative polymerase δ. This figure shows that modeled template-primer superposes extremely well onto the one reported in the cryoEM structures (template – magenta, primer – gray) [4-6]. The 3’–5’ Exonuclease domain of DNA polymerase is colored in limon color, the fingers in blue, the N-terminal in light grey, the palm in red, and insert 3 in light pink. The predicted structure of OPG148 was superposed onto the reported structure of VACV OPG148 (1-50) bound to OPG71 (PDB entry 6XZP) to obtain a relative position of OPG148 and OPG71. OPG148 is shown in forest green color. Similarly, the OPG116 was modeled through homology modelling using VACV OPG116 bound to a small segment of OPG148 as a template (PDB entry 4OD8).
See this image and copyright information in PMC

Comment on

References

    1. Yang Q, Xia D, Syed AAS, Wang Z, Shi Y. Highly accurate protein structure prediction and drug screen of monkeypox virus proteome. J Infect 2023;86:66–117. - PMC - PubMed
    1. Kannan SR, Sachdev S, Reddy AS, Kandasamy SL, Byrareddy SN, Lorson CL, et al. Mutations in the monkeypox virus replication complex: potential contributing factors to the 2022 outbreak. J Autoimmun 2022;133:102928. - PMC - PubMed
    1. Li Y, Shen Y, Hu Z, Yan R. Structural basis for the assembly of the DNA polymerase holoenzyme from a monkeypox virus variant. Sci Adv 2023;9:eadg2331. - PMC - PubMed
    1. Xu T, Zhang L. Rising prevalence of mpox in China, Japan, and Republic of Korea. J Infect 2023. 10.1016/j.jinf.2023.07.017 - DOI - PubMed
    1. Peng Q, Xie Y, Kuai L, Wang H, Qi J, Gao GF, et al. Structure of monkeypox virus DNA polymerase holoenzyme. Science 2023;379:100–5. - PubMed

MeSH terms

LinkOut - more resources