In Vitro and In Vivo Biological Assessments of 3D-Bioprinted Scaffolds for Dental Applications

Abstract

Three-dimensional (3D) bioprinting is a unique combination of technological advances in 3D printing and tissue engineering. It has emerged as a promising approach to address the dilemma in current dental treatments faced by clinicians in order to repair or replace injured and diseased tissues. The exploration of 3D bioprinting technology provides high reproducibility and precise control of the bioink containing the desired cells and biomaterial over the architectural and dimensional features of the scaffolds in fabricating functional tissue constructs that are specific to the patient treatment need. In recent years, the dental applications of different 3D bioprinting techniques, types of novel bioinks, and the types of cells used have been extensively explored. Most of the findings noted significant challenges compared to the non-biological 3D printing approach in constructing the bioscaffolds that mimic native tissues. Hence, this review focuses solely on the implementation of 3D bioprinting techniques and strategies based on cell-laden bioinks. It discusses the in vitro applications of 3D-bioprinted scaffolds on cell viabilities, cell functionalities, differentiation ability, and expression of the markers as well as the in vivo evaluations of the implanted bioscaffolds on the animal models for bone, periodontal, dentin, and pulp tissue regeneration. Finally, it outlines some perspectives for future developments in dental applications.

Keywords: bioink; cell laden; dental tissues regeneration; regenerative dentistry; three-dimensional bioprinting; tissue engineering.

Figure 1

Three-dimensional bioprinted construct for dental tissue engineering applications. Reproduced with permission from Mohd et al. (2022) [4] (http://creativecommons.org/licenses/by/4.0/) (accessed on 12 June 2023).

Figure 2

Common three-dimensional bioprinting techniques. Reproduced with permission from Gungor-Ozkerim et al. (2018) [36]; permission conveyed through Copyright Clearance Center, Inc.