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. 2023 Jul 26;11(8):1881.
doi: 10.3390/microorganisms11081881.

Whole-Genome Sequencing of Mycobacterium tuberculosis Isolates from Diabetic and Non-Diabetic Patients with Pulmonary Tuberculosis

Ranjitha Shankaregowda  1   2 , Yuan Hu Allegretti  3 , Mahadevaiah Neelambike Sumana  2 , Morubagal Raghavendra Rao  2 , Eva Raphael  4 , Padukudru Anand Mahesh  5 , Lee W Riley  1
Affiliations

Whole-Genome Sequencing of Mycobacterium tuberculosis Isolates from Diabetic and Non-Diabetic Patients with Pulmonary Tuberculosis

Ranjitha Shankaregowda et al. Microorganisms. .

Abstract

The association of tuberculosis and type 2 diabetes mellitus has been a recognized re-emerging challenge in management of the convergence of the two epidemics. Though much of the literature has studied this association, there is less knowledge in the field of genetic diversities that might occur in strains infecting tuberculosis patients with and without diabetes. Our study focused on determining the extent of diversity of genotypes of Mycobacterium tuberculosis in both these categories of patients. We subjected 55 M. tuberculosis isolates from patients diagnosed with pulmonary TB with and without type 2 diabetes mellitus to whole-genome sequencing on Illumina Hi Seq platform. The most common lineage identified was lineage 1, the Indo-Oceanic lineage (n = 22%), followed by lineage 4, the Euro-American lineage (n = 18, 33%); lineage 3, the East-African Indian lineage (n = 13, 24%); and lineage 2, the East-Asian lineage (n = 1, 2%). There were no significant differences in the distribution of lineages in both diabetics and non-diabetics in the South Indian population, and further studies involving computational analysis and comparative transcriptomics are needed to provide deeper insights.

Keywords: Mycobacterium tuberculosis; phylogenetic analysis; whole-genome sequencing.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Distribution of lineages in the study population. Major lineage identified was Indo-Oceanic (n = 22) followed by Euro-American lineage (n = 18), East-African Indian lineage (n = 13), and East Asian lineage (n = 1). One particular isolate out of the fifty-five was identified as contaminated.
Figure 2
Figure 2
A pictorial representation of lineages in different taluks and districts of South Karnataka. (Lineage 1- Green; Lineage 2- Red; Lineage 3- Pink; Lineage 4- Blue). All 4 lineages were detected in the study population from Mysore, i.e., lineage 1 (n = 11), lineage 2 (n = 1), lineage 3 (n = 4), and lineage 4 (n = 10).
Figure 3
Figure 3
Roary matrix showing distribution of core genes and accessory genes.
Figure 4
Figure 4
Phylogenetic tree constructed based on core genes. Isolates marked as (D) indicate TB-DM.
Figure 5
Figure 5
Phylogenetic tree constructed based on accessory genes. Isolates marked as (D) indicate TB-DM.
See this image and copyright information in PMC

References

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