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. 1979 Mar;9(3):205-10.
doi: 10.1002/eji.1830090307.

A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody

A J McMichael, J R Pilch, G Galfré, D Y Mason, J W Fabre, C Milstein

A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody

A J McMichael et al. Eur J Immunol. 1979 Mar.

Abstract

Spleen cells from a BALB/c mouse that had been immunized with human thymocytes were fused with the myeloma line P3-NS 1/1 Ag 4.1. One of the resulting hybrid clones (NA 1/34) secreted an antibody that was highly specific for human thymocytes. Eighty-five % of thymocytes expressed the antigen designated HTA1. There were an estimated 15 x 10(4) molecules of HTA 1 per cell, and it is therefore a major surface molecule. The expression of this antigen on thymocytes appears to be reciprocal to HLA, as recognized by another monoclonal antibody W6/32. Immunoprecipitated material from [125I]-labeled thymocyte membranes was analyzed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate which disclosed a single component of 45,000 molecular weight.

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