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. 2023 Sep 15;133(7):651-653.
doi: 10.1161/CIRCRESAHA.123.322640. Epub 2023 Aug 28.

Cardioprotective and Anti-Inflammatory Effects of FAM3D in Myocardial Ischemia-Reperfusion Injury

James Rhee  1   2 , Rebecca Freeman  1   2 , Kangsan Roh  1   2 , Margaret Lyons  1   2 , Chunyang Xiao  1 , Daniel Zlotoff  1 , Ashish Yeri  1 , Haobo Li  1 , Justin Guerra  1   3 , J Sawalla Guseh  1 , Alexandra Kuznetsov  1 , Nicholas Houstis  1 , Jason Roh  1 , Federico Damilano  1 , Xiaojun Liu  1 , Michael Silverman  1 , Raymond Kwong  4 , Saumya Das  1 , Anthony Rosenzweig  1   3
Affiliations

Cardioprotective and Anti-Inflammatory Effects of FAM3D in Myocardial Ischemia-Reperfusion Injury

James Rhee et al. Circ Res. .
No abstract available

Keywords: formyl peptide receptor; heart attack; heart failure; inflammation; ischemia-reperfusion injury; myocardial infarction; ventricular remodeling.

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Conflict of interest statement

Disclosures None.

Figures

Figure.
Figure.
A, Stratification of post-MI remodelers by change in LVESVI as assessed by serial cardiac MRI. Volcano plot showing differentially expressed proteins between adverse and favorable remodelers. B, Correlations between aptamer-based SomaScan and ELISA (Abnova) measurements of FAM13D and between plasma FAM3D and troponin-I levels 8h after cardiac reperfusion. C, Western blots of murine heart and splenic FAM3D 24h after cardiac IRI, with quantification of splenic FAM3D protein (normalized to vinculin, Image Lab software, Biorad) and RNA. n=4 mice per group. p-values calculated with Mann-Whitney test. D, Mice were injected with either adenoviral GFP (n=6) or FAM3D (n=12) (1e12 ifu/mouse) one week prior to IRI, or vehicle (n=4) or recombinant FAM3D (0.4μg/gBW, n=4) 30 minutes prior to IRI. Heart sections collected after 24h of reperfusion were quantified for areas of underperfusion lacking fluorescent microspheres (area-at-risk, AAR, grayscale images) and infarct areas (red 2,3,5-triphenyltetrazolium chloride stained images). Hearts collected after 12h of reperfusion were stained for neutrophils in red (anti-mouse Gr-1 (Ly-6G/Ly-6C)). Blue = DAPI; turquoise=mouse alpha-actinin. (Leica LAS X, ImageJ software). n=5 per group. scale bars = 1mm. E, Human peripheral neutrophils were treated with 100nM serum amyloid A and 100nM recombinant FAM3D, either alone or in combination, for 3 hours. **p<0.01, ***p<0.001 using Kruskal-Wallis test. F, Fractional shortening and M-mode echocardiographs (GE VividE90) from mice injected with recombinant FAM3D (1μg/gBW i.p., daily for three days) and adenoviral FAM3D 24h after reperfusion. n=8 per group.
See this image and copyright information in PMC

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