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Review
. 2023 Dec;120(12):3427-3445.
doi: 10.1002/bit.28540. Epub 2023 Aug 28.

Recent advances in structure-based enzyme engineering for functional reconstruction

Shen-Yuan Xu  1   2   3 , Lei Zhou  1   2   3 , Ying Xu  1   2   3 , Han-Yue Hong  1   2   3 , Chen Dai  1   2   3 , Ya-Jun Wang  1   2   3 , Yu-Guo Zheng  1   2   3
Affiliations
Review

Recent advances in structure-based enzyme engineering for functional reconstruction

Shen-Yuan Xu et al. Biotechnol Bioeng. 2023 Dec.

Abstract

Structural information can help engineer enzymes. Usually, specific amino acids in particular regions are targeted for functional reconstruction to enhance the catalytic performance, including activity, stereoselectivity, and thermostability. Appropriate selection of target sites is the key to structure-based design, which requires elucidation of the structure-function relationships. Here, we summarize the mutations of residues in different specific regions, including active center, access tunnels, and flexible loops, on fine-tuning the catalytic performance of enzymes, and discuss the effects of altering the local structural environment on the functions. In addition, we keep up with the recent progress of structure-based approaches for enzyme engineering, aiming to provide some guidance on how to take advantage of the structural information.

Keywords: biocatalysis; catalytic performance; enzyme engineering; enzyme structure; rational design; structure-function relationship.

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References

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