Rare Variant Genetics and Dilated Cardiomyopathy Severity: The DCM Precision Medicine Study

Abstract

Background: Dilated cardiomyopathy (DCM) can lead to advanced disease, defined herein as necessitating a durable left ventricular assist device or a heart transplant (LVAD/HT). DCM is known to have a genetic basis, but the association of rare variant genetics with advanced DCM has not been studied.

Methods: We analyzed clinical and genetic sequence data from patients enrolled between 2016 and 2021 in the US multisite DCM Precision Medicine Study, which was a geographically diverse, multiracial, multiethnic cohort. Clinical evaluation included standardized patient interview and medical record query forms. DCM severity was classified into 3 groups: patients with advanced disease with LVAD/HT; patients with an implantable cardioverter defibrillator (ICD) only; or patients with no ICD or LVAD/HT. Rare variants in 36 DCM genes were classified as pathogenic or likely pathogenic or variants of uncertain significance. Confounding factors we considered included demographic characteristics, lifestyle factors, access to care, DCM duration, and comorbidities. Crude and adjusted associations between DCM severity and rare variant genetic findings were assessed using multinomial models with generalized logit link.

Results: Patients' mean (SD) age was 51.9 (13.6) years; 42% were of African ancestry, 56% were of European ancestry, and 44% were female. Of 1198 patients, 347 had LVAD/HT, 511 had an ICD, and 340 had no LVAD/HT or ICD. The percentage of patients with pathogenic or likely pathogenic variants was 26.2%, 15.9%, and 15.0% for those with LVAD/HT, ICD only, or neither, respectively. After controlling for sociodemographic characteristics and comorbidities, patients with DCM with LVAD/HT were more likely than those without LVAD/HT or ICD to have DCM-related pathogenic or likely pathogenic rare variants (odds ratio, 2.3 [95% CI, 1.5-3.6]). The association did not differ by ancestry. Rare variant genetic findings were similar between patients with DCM with an ICD and those without LVAD/HT or ICD.

Conclusions: Advanced DCM was associated with higher odds of rare variants in DCM genes adjudicated as pathogenic or likely pathogenic, compared with individuals with less severe DCM. This finding may help assess the risk of outcomes in management of patients with DCM and their at-risk family members.

Registration: URL: https://www.

Clinicaltrials: gov; Unique identifier: NCT03037632.

Keywords: cardiomyopathy, dilated; genetics; risk.

Figure 1.

DCM Precision Medicine Study recruitment and analysis.

Figure 2.. Most deleterious DCM-related rare variant by DCM severity status.

This figure presents the percent distributions of patients by their most deleterious DCM-related rare variant (P/LP, VUS only, or negative) and DCM severity status (LVAD/HT, ICD only, or neither). The percentage of patients with P/LP variants was higher for those with LVAD/HT compared with those with ICD and none (26.2%, 15.9%, and 15.0%, respectively, Ps<0.001). Abbreviations: ICD=Implantable cardioverter-defibrillator. LVAD=Left ventricular assist device. P/LP=Pathogenic/Likely pathogenic. VUS=Variant of uncertain significance.