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. 2023:2704:143-156.
doi: 10.1007/978-1-0716-3385-4_8.

Fungal Sterol Analyses by Gas Chromatography-Mass Spectrometry Using Different Derivatives

Aurélie Mossion  1 , Isabelle Ourliac-Garnier  2 , Gaetane Wielgosz-Collin  3
Affiliations

Fungal Sterol Analyses by Gas Chromatography-Mass Spectrometry Using Different Derivatives

Aurélie Mossion et al. Methods Mol Biol. 2023.

Abstract

Sterols are the main components of the fungal membrane. Their study can be used to describe the chemical biodiversity among the strains and species or to work on antifungal treatment. Those molecules can be analyzed by gas chromatography coupled with mass spectrometry (GC-MS) as free molecules or after derivation as acetate or trimethylsilyl ether (TMSi). Here we describe how to extract sterols from fungal biomass according to its physiological form and the culture conditions (liquid and solid media). The different methodologies that can be used to obtain free sterols, acetate, and/or TMSi derivatives are presented. Identification keys using the fragmentation at 70 eV are also described.

Keywords: Acetate; Extraction; Fungal sterols; GC–MS; Identification; Saponification; TMS derivatives.

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