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. 2023:2704:231-243.
doi: 10.1007/978-1-0716-3385-4_14.

Scale-Up of Phytosterols Bioconversion into Androstenedione

Sonia Martínez-Cámara  1 , Manuel de la Torre  1 , José-Luis Barredo  1 , Marta Rodríguez-Sáiz  2
Affiliations

Scale-Up of Phytosterols Bioconversion into Androstenedione

Sonia Martínez-Cámara et al. Methods Mol Biol. 2023.

Abstract

Phytosterols, coming as a by-product of vegetable oils or wood pulp, contain the cyclopentanoperhydrophenanthrene nucleus and can be bioconverted into steroid intermediates by removing the C17 side chain. This chapter shows the scale-up, from flask to bioreactor, of phytosterols bioconversion into 4-androstene-3,17-dione (androstenedione; AD) using Mycolicibacterium neoaurum B-3805. Due to the fact that phytosterols and AD are nearly insoluble in water, two-phase systems and the use of chemically modified cyclodextrins have been described as methods to solve it. Here, we use a water-oil two-phase system that allows the bioconversion of up to 20 g/L of phytosterols into AD in 5 L and 20 L bioreactors.

Keywords: 4-Androstene-3,17-dione; AD; Androstenedione; Bioconversion; Fermentation; Mycobacterium neoaurum; Mycolicibacterium neoaurum; Phytosterols; Two-phase system.

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