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. 2023 Aug 29;16(1):188.
doi: 10.1186/s13104-023-06462-4.

Optimization of the arginase activity assay micromethod for macrophages and sera

Romaric Nzoumbou-Boko  1 , Cyrille Oliver Ozzin-Kholy Zolipou  2   3 , Brice Martial Yambiyo  4 , Silla Semballa  5 , Mireille Cornelia Ingrid Denissio Morissi Nalingbo  2 , Sylvie Daulouède  3 , Philippe Vincendeau  3
Affiliations

Optimization of the arginase activity assay micromethod for macrophages and sera

Romaric Nzoumbou-Boko et al. BMC Res Notes. .

Abstract

Objective: We optimized the spectrophotometric micromethod for the determination of arginase activity based on the Corraliza et al. modification of Schimke's method. Arginase activity in sera from patients suffering from human African trypanosomiasis, in macrophage lysates from trypanosome-infected mice, and in purified bovine liver arginase was compared using the conventional and optimized micromethods.

Results: The sensitivity of both micromethods was comparable. However, our optimized method has the following advantages: it uses small sample volumes (6 µl per assay vs. 50 µl) and reagent volumes (200 µl vs. 400 µl), it can be carried out in a single microplate well, thereby minimizing handling, and it requires fewer materials and utilizes readily available equipment. Our optimized method proved to be applicable and well suited for small-volume samples and resource-poor laboratories.

Keywords: Arginase activity; Micromethod optimization; Trypanosomiasis.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Enzymatic Assay of Arginase with two procedures
Fig. 2
Fig. 2
Use of the non-parametric Mann-Whitney test for intergroup comparisons between activated macrophages vs. control macrophages and HAT sera versus control sera. For the comparison of the two methods, the Kruskal-Wallis test is used for determination of arginase activity in macrophage mouse controls (n = 7) and macrophage mouse T. musculi infected (n = 7)
Fig. 3
Fig. 3
Use of the non-parametric Mann-Whitney test for intergroup comparisons between activated macrophages vs. control macrophages and HAT sera versus control sera. For the comparison of the two methods, the Kruskal-Wallis test is used for determination of arginase activity in sera from controls (n = 10) and patients with human African trypanosomiasis (n = 10) from the Bouenza focus in the Republic of Congo
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References

    1. -Caldwell RW, Rodriguez PC, Toque HA, Narayanan SP, Caldwell RB. Arginase: a multifaceted enzyme important in Health and Disease. Physiol Rev. 2018;98(2):641–65. doi: 10.1152/physrev.00037.2016. - DOI - PMC - PubMed
    1. -Nzoumbou-Boko R, Dethoua M, Gabriel F, Buguet A, Cespuglio R, Courtois P, Daulouède S, Bouteille B, Ngampo S, Mpandzou G, Semballa S, Vincendeau P. Serum arginase, a biomarker of treatment efficacy in human african trypanosomiasis. J Clin Microbiol. 2013;51(7):2379–81. doi: 10.1128/JCM.03371-12. - DOI - PMC - PubMed
    1. -Cloke TE, Garvey L, Choi BS, Abebe T, Hailu A, Hancock M, Kadolsky U, Bangham CRM, Munder M, Müller I, Taylor GP, Kropf P. Increased level of arginase activity correlates with disease severity in HIV-seropositive patients. J Infect Dis. 2010;202(3):374–85. doi: 10.1086/653736. - DOI - PMC - PubMed
    1. -Kupani M, Sharma S, Pandey RK, Kumar R, Sundar S, Mehrotra S. IL-10 and TGF-β Induced Arginase expression contributes to deficient nitric oxide response in human visceral leishmaniasis. Front Cell Infect Microbiol. 2021;10:614165. doi: 10.3389/fcimb.2020.614165. - DOI - PMC - PubMed
    1. -Derakhshani A, Hemmat N, Asadzadeh Z, Ghaseminia M, Shadbad MA, Jadideslam G, Silvestris N, Racanelli V, Baradaran B. Arginase 1 (Arg1) as an Up-Regulated gene in COVID-19 patients: a promising marker in COVID-19 Immunopathy. J Clin Med. 2021;10(5):1051. doi: 10.3390/jcm10051051. - DOI - PMC - PubMed

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