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. 2023 Aug 25;28(4):e2322277.
doi: 10.1590/2177-6709.28.4.e2322277.oar. eCollection 2023.

Assessment of dehydroepiandrosterone sulphate (DHEAS) and cortisol levels in saliva and their correlation to cervical vertebrae maturation method in males and females at different growth stages: a clinical study

Nikita Dattatraya Gavate  1 , Smitha Shetty  1 , Rabindra S Nayak  1 , Vinay K  1 , Anjali Narayan  1 , Chaitra K R  1
Affiliations

Assessment of dehydroepiandrosterone sulphate (DHEAS) and cortisol levels in saliva and their correlation to cervical vertebrae maturation method in males and females at different growth stages: a clinical study

Nikita Dattatraya Gavate et al. Dental Press J Orthod. .

Abstract
in English, Portuguese

Objective: The aim of this in-vivo study was to assess the salivary dehydroepiandrosterone sulphate (DHEAS) and cortisol levels, and their correlation to the Cervical Vertebrae Maturation method (CVM) in males and females at pre-pubertal, pubertal and post-pubertal growth stages.

Methods: 48 patients (24 males, 24 females) who were to undergo routine orthodontic treatment were screened according to the inclusion and exclusion criteria. Then subjects were grouped according to CVM stages, using lateral cephalogram, in pre-pubertal, pubertal and post-pubertal groups. Unstimulated saliva from the selected subjects was collected. DHEAS and cortisol levels in the salivary samples were estimated by Enzyme-Linked Immunosorbent assay (ELISA). Then they were compared to Cervical Vertebrae Maturation Method stages. One-way ANOVA test followed by Tukey's post-hoc test was used to compare the salivary DHEAS and cortisol levels between different CVM stages in males and females. Independent Student t-test was used to compare the mean salivary DHEAS and cortisol levels between different males and females in each CVM stage.

Result: There was a progressive increase in salivary DHEAS and cortisol concentration as skeletal maturation progressed from CVM stages 1 and 2, CVM stages 3 and 4, reaching the highest value at CVM stages 5 and 6. Their levels were higher in males than females.

Conclusion: The salivary DHEAS and cortisol levels can be useful as a potential indicator of skeletal maturation, to aid in the assessment of pubertal status.

Objetivo:: O objetivo deste estudo in vivo foi avaliar os níveis salivares de sulfato de dehidroepiandrosterona (DHEAS) e de cortisol, e sua correlação com o método de maturação das vértebras cervicais (CVM) em homens e mulheres nas fases de crescimento pré-puberal, puberal e pós-puberal.

Métodos:: 48 pacientes (24 homens, 24 mulheres) que se submeteriam a tratamento ortodôntico de rotina foram selecionados de acordo com os critérios de inclusão e exclusão. Em seguida, usando telerradiografia lateral, os indivíduos foram agrupados de acordo com os estágios CVM, em grupos pré-puberal, puberal e pós-puberal. Foi feita coleta de saliva não estimulada nos indivíduos selecionados. Os níveis de DHEAS e cortisol nas amostras salivares foram avaliados pelo teste ELISA (Enzyme-Linked Immunosorbent assay). Em seguida, foram comparados aos estágios do método CVM. O teste ANOVA de uma via seguido pelo teste post-hoc de Tukey foi usado para comparar o DHEAS salivar e os níveis de cortisol entre os diferentes estágios de CVM em homens e mulheres. O teste t de Student independente foi usado para comparar a média de DHEAS salivar e os níveis de cortisol entre diferentes homens e mulheres em cada estágio de CVM.

Resultados:: Houve um aumento progressivo no DHEAS salivar e na concentração de cortisol à medida que a maturação esquelética progrediu dos estágios CVM 1 e 2, para os estágios CVM 3 e 4, atingindo o valor mais alto nos estágios CVM 5 e 6. Seus níveis foram maiores nos homens do que nas mulheres.

Conclusões:: Os níveis salivares de DHEAS e cortisol podem ser úteis como um potencial indicador de maturação esquelética, para auxiliar na avaliação do estado puberal.

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Conflict of interest statement

The authors report no commercial, proprietary or financial interest in the products or companies described in this article.

Figures

Figure 1:
Figure 1:. Mean salivary DHEAS levels ( in pg/ml ) between different CVM stages.
Figure 2:
Figure 2:. Mean salivary cortisol levels ( in pg/ml ) between different CVM stages.
See this image and copyright information in PMC

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References

    1. Baccetti T, Franchi L, Toth LR, McNamara JA., Jr Treatment timing for Twin-block therapy. Am J Orthod Dentofacial Orthop. 2000;118(2):159–170. - PubMed
    1. Hagg U, Taranger J. Maturation indicators and the pubertal growth spurt. Am J Orthod. 1982;82(4):299–309. - PubMed
    1. Engström C, Engström H, Sagne S. Lower third molar development in relation to skeletal maturity and chronological age. Angle Orthod. 1983;53(2):97–106. - PubMed
    1. Coutinho S. Relationships between mandibular canine calcification stages and skeletal maturity. Am J Orthod Dentofacial Orthop. 1993;104(3):262–268. - PubMed
    1. Lamparski DG. Skeletal age assessment utilizing cervical vertebrae. Am J Orthod. 1975;67(4):458–459.

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