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. 2023 Dec:48:101124.
doi: 10.1016/j.cbd.2023.101124. Epub 2023 Aug 22.

Tandem mass tag (TMT) quantitative proteomics and phosphoproteomic of Takifugu rubripes infected with Cryptocaryon irritans

Yi Yang  1 , Yanyun Liu  1 , Yuqing Xia  2 , Jianxin Cheng  3 , Pengfei Liu  4
Affiliations

Tandem mass tag (TMT) quantitative proteomics and phosphoproteomic of Takifugu rubripes infected with Cryptocaryon irritans

Yi Yang et al. Comp Biochem Physiol Part D Genomics Proteomics. 2023 Dec.

Abstract

In this study, we identified the differentially expressed proteins in gills stimulated by infected ciliates and analyzed the immune mechanisms of T. rubripes infected with the ciliate Cryptocaryon irritans. Through liquid chromatography analysis, a total of 144 proteins were identified with significant differences, of which 58 were upregulated and 86 were downregulated. Among phosphorylated proteins, we identified a total of 167 significantly different phosphorylated proteins, of which 44 were upregulated, 123 were downregulated, 60 were upregulated, and 208 were downregulated. We analyzed the data of proteomics and Phosphorylated proteome quantification protein omics to finally identify three phosphorylated proteins (RPS27, eNOS and CaM) and two phosphorylated protein kinases(CaMKII and MAPK1) as potential biomarkers for T. rubripes immune responses. We finally identified three phosphorylated proteins (RPS27, eNOS and CaM) and two phosphorylated protein kinases (CaMKII and MAPK1) as potential biomarkers of immune response of T. rubripes. Our research findings provide new insights into the immune mechanism of T. rubripes, which may serve as an effective indicator of C. irritans infection in T. rubripes.

Keywords: Cryptocaryon irritans; Phosphoproteomic; Proteomics; Takifugu rubripes.

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Conflict of interest statement

Declaration of competing interest We could like to submit the enclosed manuscript entitled “Tandem mass tag (TMT) quantitative Proteomics and phosphoproteomic analysis of Takifugu rubripes infected with Cryptocaryon irritans”. No conflict of interest exists in the submission of this manuscript, and manuscript is approved by all authors for publication. I would like to declare that this article has not been published previously, and not under consideration for publication elsewhere, in whole or in part.

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