Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 May;202(5):2042-2051.
doi: 10.1007/s12011-023-03826-6. Epub 2023 Aug 30.

The Effects of Zinc Oxide Nanoparticles on Antioxidation, Inflammation, Tight Junction Integrity, and Apoptosis in Heat-Stressed Bovine Intestinal Epithelial Cells In Vitro

Yuanxiao Li  1 , Mengying Pan  1 , Sudan Meng  1 , Wenhao Xu  1 , Shuai Wang  1 , Mengying Dou  2   3 , Cai Zhang  4
Affiliations

The Effects of Zinc Oxide Nanoparticles on Antioxidation, Inflammation, Tight Junction Integrity, and Apoptosis in Heat-Stressed Bovine Intestinal Epithelial Cells In Vitro

Yuanxiao Li et al. Biol Trace Elem Res. 2024 May.

Abstract

Zinc oxide nanoparticles (nano-ZnO) have diverse applications in numerous biomedical processes. The present study explored the effects of these nanoparticles on antioxidation, inflammation, tight junction integrity, and apoptosis in heat-stressed bovine intestinal epithelial cells (BIECs). Primary BIECs that were isolated and cultured from calves either were subjected to heat stress alone (42°C for 6 h) or were simultaneously heat-stressed and treated with nano-ZnO (0.8 μg/mL). Cell viability, apoptosis, and expression of genes involved in antioxidation (Nrf2, HO-1, SOD1, and GCLM), inflammation-related genes (TLR4, NF-κB, TNF-α, IL-6, IL-8, and IL-10), intestinal barrier genes (Claudin, Occludin, and ZO-1), and apoptosis-related genes (Cyt-c, Caspase-3, and Caspase-9) were assessed to evaluate the effect of nano-ZnO on heat-stressed BIECs. The nanoparticles significantly increased cell viability and decreased the rate of apoptosis of BIECs induced by heat stress. In addition, nano-ZnO promoted the expression of antioxidant-related genes HO-1 and GCLM and anti-inflammatory cytokine gene IL-10, and inhibited the pro-inflammatory cytokine-related genes IL-6 and IL-8. The nanoparticles also enhanced expression of the Claudin and ZO-1 genes, and decreased expression of the apoptosis-related genes Cyt-c and Caspase-3. These results reveal that nano-ZnO improve the antioxidant and immune capacity of BIECs and mitigate apoptosis of intestinal epithelial cells induced by heat stress. Thus, nano-ZnO have potential for detrimental the adverse effects of heat stress in dairy cows.

Keywords: Antioxidation; Bovine intestinal epithelial cells; Heat stress; Inflammation; Nano-ZnO; Tight junction.

PubMed Disclaimer

References

    1. Zheng Y, Xie T, Li S, Wang W, Wang Y, Cao Z, Yang H (2022) Effects of selenium as a dietary source on performance, inflammation, cell damage, and reproduction of livestock induced by heat stress: a review. Front Immunol 12:820853. https://doi.org/10.3389/fimmu.2021.820853 - DOI - PubMed - PMC
    1. Gershoni M (2023) Transgenerational transmission of environmental effects in livestock in the age of global warming. Cell Stress Chaperones. https://doi.org/10.1007/s12192-023-01325-0
    1. Cartwright SL, McKechnie M, Schmied J, Livernois AM, Mallard BA (2021) Effect of in-vitro heat stress challenge on the function of blood mononuclear cells from dairy cattle ranked as high, average and low immune responders. BMC Vet Res 17(1):233. https://doi.org/10.1186/s12917-021-02940-8.c - DOI - PubMed - PMC
    1. Li L, Tan H, Zou Z, Gong J, Zhou J, Peng N, Su L, Maegele M, Cai D, Gu Z (2020) Preventing necroptosis by scavenging ROS production alleviates heat stress-induced intestinal injury. Int J Hyperthermia 37(1):517–530. https://doi.org/10.1080/02656736.2020.1763483 - DOI - PubMed
    1. Sammad A, Umer S, Shi R, Zhu H, Zhao X, Wang Y (2020) Dairy cow reproduction under the influence of heat stress. J Anim Physiol Anim Nutr (Berl) 104(4):978–986. https://doi.org/10.1111/jpn.13257 - DOI - PubMed

LinkOut - more resources