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Comment
. 2023 Aug 31;142(9):755-756.
doi: 10.1182/blood.2023021309.

A viable alternative for editor delivery

Feyisayo Eweje  1   2 , Daniel E Bauer  3
Affiliations
Comment

A viable alternative for editor delivery

Feyisayo Eweje et al. Blood. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

Conflict-of-interest disclosure: D.E.B. is an inventor of patents related to therapeutic gene editing and has consulted for Kytopen. F.E. declares no competing financial interests.

Figures

None
Electroporation-mediated delivery of gene-editing agents is associated with cytotoxicity. In their work, Vavassori et al demonstrate that ex vivo LNP-mediated delivery of gene editors to T cells and HSPCs results in lower cytotoxicity, reduced DNA damage responses, and higher cell yield.
See this image and copyright information in PMC

Comment on

References

    1. Vavassori V, Ferrari S, Beretta S, et al. Lipid nanoparticles allow efficient and harmless ex vivo gene editing of human hematopoietic cells. Blood. 2023;142(9):812–826. - PMC - PubMed
    1. Frangoul H, Altshuler D, Cappellini MD, et al. CRISPR-Cas9 gene editing for sickle cell disease and β-thalassemia. N Engl J Med. 2021;384(3):252–260. - PubMed
    1. Stadtmauer EA, Fraietta JA, Davis MM, et al. CRISPR-engineered T cells in patients with refractory cancer. Science. 2020;367(6481):eaba7365. - PMC - PubMed
    1. Batista Napotnik T, Polajžer T, Miklavčič D. Cell death due to electroporation—a review. Bioelectrochemistry. 2021;141 - PubMed
    1. El-Kharrag R, Berckmueller KE, Madhu R, et al. Efficient polymer nanoparticle-mediated delivery of gene editing reagents into human hematopoietic stem and progenitor cells. Mol Ther. 2022;30(6):2186–2198. - PMC - PubMed