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. 2023 Sep-Oct;37(5):2161-2165.
doi: 10.21873/invivo.13314.

Detection of 14 High-risk Human Papillomavirus (HPV) Genotypes Within the Italian Cervical Cancer Screening

Maria Cenci  1 , Francesca Rossi  2 , Tiziana Pisani  2
Affiliations

Detection of 14 High-risk Human Papillomavirus (HPV) Genotypes Within the Italian Cervical Cancer Screening

Maria Cenci et al. In Vivo. 2023 Sep-Oct.

Abstract

Background/aim: The identification of high-risk human papillomavirus (HR HPV) genotypes is important both for epidemiological purposes and because the persistence of an HPV infection with the same genotype is a necessary condition for the development of cervical cancer. The purpose of this study was to analyze the prevalence of HR HPV genotypes in women enrolled in the national program for cervical cancer screening in Lazio Region, Italy.

Patients and methods: From April to November 2022, we evaluated 30,445 samples using the Anyplex TM II HPV HR Detection test (Seegene), which identifies 14 HR HPV: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68. The data were analyzed using the SG STATS platform.

Results: In total, 4,244 samples tested positive (13.9%); 3,290 samples (77.5%) were positive for one of the genotypes tested, and 954 (22.5%) were positive for more than one HPV genotype. The total prevalence (considering both single infection and co-infections) of the different genotypes was: HPV 16 755 cases (13.8%), HPV 31 704 (12.9%), HPV 68 580 (10.6%), HPV 66 436 (8.0%), HPV 52 413 (7.5%), HPV 58 411 (7.5%), HPV 51 400 (7.3%), HPV 56 366 (6.7%), HPV 39 293 (5.3%), HPV 59 260 (4.8%), HPV 45 231 (4.2%), HPV 33 230 (4.2%), HPV 18 222 (4.0%), HPV 35 173 (3.2%). Our results indicate that HPV 16 and 31 are the most prevalent genotypes in the Lazio region followed by HPV 68, 66, 52, 58, and 51.

Conclusion: The extended genotyping test allows a better risk stratification and the identification of multiple HPV infections.

Keywords: HPV prevalence; High risk human papillomavirus; extended HR HPV genotyping; national cervical cancer screening.

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Conflict of interest statement

All the Authors report no conflicts of interest in relation to this study.

Figures

Figure 1
Figure 1. Positive cases.
See this image and copyright information in PMC

References

    1. Bonde J, Bottari F, Iacobone AD, Cocuzza CE, Sandri MT, Bogliatto F, Khan KS, Ejegod DM, Gary DS, Andrews JC. Human papillomavirus same genotype persistence and risk: a systematic review. J Low Genit Tract Dis. 2021;25(1):27–37. doi: 10.1097/LGT.0000000000000573. - DOI - PMC - PubMed
    1. Okunade KS. Human papillomavirus and cervical cancer. J Obstet Gynaecol. 2020;40(5):602–608. doi: 10.1080/01443615.2019.1634030. - DOI - PMC - PubMed
    1. Bonde JH, Sandri MT, Gary DS, Andrews JC. Clinical utility of human papillomavirus genotyping in cervical cancer screening: a systematic review. J Low Genit Tract Dis. 2020;24(1):1–13. doi: 10.1097/LGT.0000000000000494. - DOI - PMC - PubMed
    1. Demarco M, Hyun N, Carter-Pokras O, Raine-Bennett TR, Cheung L, Chen X, Hammer A, Campos N, Kinney W, Gage JC, Befano B, Perkins RB, He X, Dallal C, Chen J, Poitras N, Mayrand MH, Coutlee F, Burk RD, Lorey T, Castle PE, Wentzensen N, Schiffman M. A study of type-specific HPV natural history and implications for contemporary cervical cancer screening programs. EClinicalMedicine. 2020;22:100293. doi: 10.1016/j.eclinm.2020.100293. - DOI - PMC - PubMed
    1. Smelov V, Elfström KM, Johansson AL, Eklund C, Naucler P, Arnheim-Dahlström L, Dillner J. Long-term HPV type-specific risks of high-grade cervical intraepithelial lesions: A 14-year follow-up of a randomized primary HPV screening trial. Int J Cancer. 2015;136(5):1171–1180. doi: 10.1002/ijc.29085. - DOI - PubMed

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