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. 2023 Dec;54(4):3221-3230.
doi: 10.1007/s42770-023-01109-9. Epub 2023 Aug 31.

Genetic diversity of adenovirus in neotropical bats from Brazil

Laís Santos Rizotto  1 , Larissa Mayumi Bueno  2 , Thaís Camilo Corrêa  2 , Maria Vitória Dos Santos de Moraes  1 , Amanda de Oliveira Viana  3 , Laura Morais Nascimento Silva  1 , Julia Cristina Benassi  2 , Guilherme Pereira Scagion  3 , Bruna Larotonda Telezynski Lopes  3 , Isabela Barbosa de Assis  3 , Tatiana Ometto  3 , Erick Gustavo Dorlass  3 , Irineu Noberto Cunha  4 , Ramiro Dário Melinski  5 , Gabriel Lins Leitão  4 , Roberta Costa Rodrigues  6 , Iolanda Maria da Silva Pereira  6 , Lilia D'ark Nunes Dos Santos  6 , Erika Hingst-Zaher  4 , Severino Mendes de Azevedo Junior  6 , Wallace Rodrigues Telino Junior  7 , Jansen de Araújo  3 , Edison Luiz Durigon  3 , Clarice Weis Arns  8 , Helena Lage Ferreira  9   10
Affiliations

Genetic diversity of adenovirus in neotropical bats from Brazil

Laís Santos Rizotto et al. Braz J Microbiol. 2023 Dec.

Abstract

Bats can harbor a diversity of viruses, such as adenovirus. Ten different species of bat adenoviruses (BtAdV A to J) have been previous described worlwide. In Brazil, BtAdV was described in three species of phyllostomid species: Artibeus lituratus, Desmodus rotundus, and Sturnira lilium. There are around 180 bat species in Brazil, with 67% inhabiting the Atlantic Forest, with few information about the circulation of BtAdV in this biome. We aimed to describe the molecular detection and the phylogenetic characterization and suggest a classification of BtAdVs circulating in bats from the Brazilian Atlantic Forest. We collected 382 oral and rectal swabs from 208 bats between 2014-2015 and 2020-2021 from São Paulo, Pernambuco, and Santa Catarina Brazilian states. The adenovirus detection was done by a nested PCR targeting the DNA polymerase gene, and all positive samples were sequenced by the Sanger method. The phylogenetic analyses were based on the amino acid sequences using the MEGA 7 and BEAST software. We obtained 16 positive animals (detection rate 7.7%) belonging to seven bat species: Artibeus lituratus, Carollia perspicillata, Sturnira lilium, Molossus molossus, and the first record of Phyllostomus discolor, Eptesicus diminutus, and Myotis riparius. The phylogenetic analysis based on partial amino acid sequences showed that all obtained AdV sequences belong to the Mastadenovirus genus. We observed a high genetic diversity of BtAdV and identified eleven potential BtAdV species circulating in Brazil (BtAdV K to U). Our results contribute to the epidemiological surveillance of adenovirus, increasing the knowledge about the viral diversity and the distribution of AdV in bats from the Atlantic Forest.

Keywords: Bat mastadenovirus; New species; Viral diversity; Virus surveillance.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Geographic map indicating the capture sites in the Brazilian states of Pernambuco, São Paulo, and Santa Catarina. A red dot represents the sampling sites, and the different conditions of vegetation are represented by different colors: forest in dark green; non-forest natural formation in light green; agricultural in light yellow; area without vegetation in light pink
Fig. 2
Fig. 2
Phylogenetic tree based on amino acid sequences of adenovirus DNA polymerase was generated using the Maximum Likelihood method with the Le Gascuel model and a discrete Gamma distribution (LG+G), totalizing 73 positions in the final dataset. Sequences detected in this study are highlighted in bold, and the groups are colored according to the proposed species classifications. Values lower than 70% were hidden
Fig. 3
Fig. 3
Phylogenetic tree based on amino acid sequences of adenovirus DNA polymerase was generated using the Bayesian method with Le Gascuel model distribution (LG). Sequences detected in this study are highlighted in bold, and the groups are colored according to the proposed species classifications. Values lower than 70% were hidden
See this image and copyright information in PMC

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