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. 2023 Jun 30;34(2):163-171.
doi: 10.31138/mjr.34.2.163. eCollection 2023 Jun.

Evaluation of NADPH Oxidase (NOX) Activity by nitro Blue Tetrazolium (NBT) Test in SLE Patients

Ritasman Baisya  1 , Shiva Krishna Katkam  2 , Sreejitha Ks  1 , Phani Kumar Devarasetti  1 , Vijay Kumar Kutala  1 , Liza Rajasekhar  1
Affiliations

Evaluation of NADPH Oxidase (NOX) Activity by nitro Blue Tetrazolium (NBT) Test in SLE Patients

Ritasman Baisya et al. Mediterr J Rheumatol. .
No abstract available

Keywords: BILAG score; Reactive oxygen species (ROS); SLE; SLEDAI score; nitro-blue tetrazolium (NBT).

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1.
Figure 1.
Line chart – basal & stimulated NBT in case and control.
Figure 2.
Figure 2.
Kendall–Tau matrix - SLEDAI, m-SLEDAI (Mexican -SLEDAI/clinical SLEDAI) are strongly associated with higher basal and ΔNBT (Del OD) values but not with stimulated NBT values. High dose steroid: >−10 mg prednisolone or equivalent/day. Other medications include any steroid sparing immunosuppressive – azathioprine, cyclophosphamide, mycophenolate, etc. The matrix represents correlation coefficients of pairwise variables. The red background depicts inverse association, while the green background depicts positive association.
Figure 3.
Figure 3.
Comparison of basal, stimulated NBT reduction for each category (A, B, C, D, E) of renal BILAG domain.
Figure 4.
Figure 4.
Age, gender, co-morbidities, high steroid dose, other medications, leukopenia, dsDNA, past infections explain 21.6% variability in ΔNBT. The association of ROS with using demographic and clinical variables, multiple linear regression analysis was performed. Age, gender, comorbidities, high steroid dose, other medications, leukopenia, dsDNA, past infections explained 21.6% variability in ΔNBT (increment in ROS levels).
Supplementary Figure 1s.
Supplementary Figure 1s.
Analysis of neutrophil Purity by flow cytometry. A forward-scatter versus side scatter dot plot was used to evaluate neutrophil purity.
Supplementary Figure 2s.
Supplementary Figure 2s.
NBT reduction with different cell numbers (1, 2, 4, 8, and 16 lackh cells/well without and with PMA.
Supplementary Figure 3s.
Supplementary Figure 3s.
OD value (595 nm) with different neutrophil count (with and without PMA).
Supplementary Figure 4s.
Supplementary Figure 4s.
Kendall-Tau correlation matrix. The matrix represents correlation coefficients of pair-wise variables. The red background depicts inverse association while the green background depicts positive association. NBT basal levels and ΔNBT values are not influenced by age, gender, total leukocyte count, neutrophil count, lymphocyte count, hemoglobin, and platelet count. However, high steroid dose increases basal NBT and ΔNBT values while comorbidities decrease the basal and ΔNBT. The SLEDAI, m-SLEDAI and disease activity are strongly associated with higher basal and ΔNBT values. Stimulated NBT values are not affected by demographic, haematological, and clinical variables investigated.
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