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. 2023;15(2):106-115.
doi: 10.34172/jcvtr.2023.31719. Epub 2023 Jun 29.

The effects of vitamin D on cardiovascular damage induced by lipopolysaccharides in rats

Narges Marefati  1   2 , Farimah Beheshti  3   4 , Akbar Anaeigoudari  5 , Fatemeh Alipour  6 , Reyhaneh Shafieian  6 , Fatemeh Akbari  7 , Maryam Pirasteh  8 , Maryam Mahmoudabady  2   7 , Hossein Salmani  9 , Sara Mawdodi  10 , Mahmoud Hosseini  7   11
Affiliations

The effects of vitamin D on cardiovascular damage induced by lipopolysaccharides in rats

Narges Marefati et al. J Cardiovasc Thorac Res. 2023.

Abstract

Introduction: Inflammation and oxidative stress are contributed to cardiovascular diseases. Vitamin D (Vit D) has antioxidant and anti-inflammatory properties. In the current research, the effect of Vit D on cardiac fibrosis and inflammation, and oxidative stress indicators in cardiovascular tissues was studied in lipopolysaccharides(LPS) injected rats.

Methods: Rats were distributed into 5 groups and were treated for 2 weeks. Control: received vehicle(saline supplemented with tween-80) instead of Vit D and saline instead of LPS, LPS: treated by 1 mg/kg of LPS and was given vehicle instead of Vit D, LPS-Vit D groups: received 3 doses of Vit D (100, 1000, and 10000 IU/kg) of Vit D in addition to LPS. Vit D was dissolved in saline supplemented with tween-80 (final concentration 0.1%) and LPS was dissolved in saline. The white blood cell (WBC) was counted. Oxidative stress markers were determined in serum, aorta, and heart. Cardiac tissue fibrosis was also estimated using Masson's trichrome staining method.

Results: WBC and malondialdehyde (MDA) were higher in the LPS group than the control group, whereas the thiol content, superoxide dismutase (SOD), and catalase (CAT) were lower in the LPS group than the control group (P<0.01 and P<0.001). Administration of Vit D decreased WBC (P<0.001) and MDA (P<0.05 and P<0.001) while enhanced thiol (dose 10000 IU/Kg) (P<0.001), SOD (dose 10000 IU/kg) (P<0.001), and CAT (P<0.05 and P<0.001) compared to the LPS group. All doses of Vit D also decreased cardiac fibrosis compared to the LPS group (P<0.001).

Conclusion: Vit D protected the cardiovascular against the detrimental effect of LPS. This cardiovascular protection can be attributed to the antioxidant and anti-inflammatory properties of Vit D.

Keywords: Cardiovascular; Fibrosis; Inflammation; Lipopolysaccharide; Oxidative Stress; Vitamin D.

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Conflict of interest statement

There is no conflict of interest to declare.

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