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. 2024 Feb;48(1):271-278.
doi: 10.1007/s11259-023-10192-0. Epub 2023 Sep 1.

Evidence of Mycobacterium avium subsp. paratuberculosis binding to albumin: technical and biological implications

Ana Jolly  1 , María Laura Barnech  2   3 , Juan José Duarte  2 , Jorge Suhevic  4 , Ana María Jar  2 , Silvia Leonor Mundo  2   5
Affiliations

Evidence of Mycobacterium avium subsp. paratuberculosis binding to albumin: technical and biological implications

Ana Jolly et al. Vet Res Commun. 2024 Feb.

Abstract

Albumin binding ability is a well-characterized feature of many bacteria. To the best of our knowledge, there are no previous reports about this ability among mycobacteria, even when bovine serum albumin (BSA) is a common component of supplements used for the enrichment of synthetic media for mycobacterial growth in vitro and also of buffers used in laboratory techniques. In this work we explored the albumin binding ability of Mycobacterium avium subsp. paratuberculosis (MAP), a pathogenic bacterium causing a known and relevant ruminant disease worldwide, by immunizing rabbits with MAP (grown in media containing or not BSA) or BSA and conducting ELISA and immunoblot experiments with the obtained sera. As a result, we found that MAP can bind BSA when cultured in a conventional BSA-containing medium and when incubated for a short time in the presence of the protein. We also evaluated the host specificity of MAP interaction with albumin and found a preference for the protein of bovine origin when compared with its horse and rabbit homologs. Considerations about its technical and biological implications are discussed.

Keywords: Albumin binding ability; BSA; Mycobacteria; Paratuberculosis.

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