Increased intracellular miR-142 in adults with Type 1 diabetes

Li Huang¹, Andrzej S Januszewski², Yusuke Takahashi³, David N O'Neal⁴, Jian-Xing Ma⁵, Alicia J Jenkins⁶

Affiliations

¹ Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Department of Ophthalmology, Fujian Medical University Union Hospital, Fuzhou, Fujian Province, China.
² NHMRC Clinical Trials Centre, University of Sydney, Sydney, NSW, Australia; Deptartment of Medicine, University of Melbourne, Melbourne, VIC, Australia; Sydney Pharmacy School, University of Sydney, Sydney, NSW, Australia.
³ Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Deptartment of Biochemistry, Wake Forest University, School of Medicine, Winton-Salem, NC, USA. Electronic address: ytakahas@wakehealth.edu.
⁴ NHMRC Clinical Trials Centre, University of Sydney, Sydney, NSW, Australia; Deptartment of Medicine, University of Melbourne, Melbourne, VIC, Australia; Deptartment of Biochemistry, Wake Forest University, School of Medicine, Winton-Salem, NC, USA.
⁵ Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Deptartment of Biochemistry, Wake Forest University, School of Medicine, Winton-Salem, NC, USA; Baker Heart and Diabetes Institute, Melbourne, VIC, Australia.
⁶ NHMRC Clinical Trials Centre, University of Sydney, Sydney, NSW, Australia; Deptartment of Medicine, University of Melbourne, Melbourne, VIC, Australia; Deptartment of Endocrinology and Diabetes, St. Vincent's Hospital, Melbourne, VIC, Australia; Baker Heart and Diabetes Institute, Melbourne, VIC, Australia. Electronic address: alicia.jenkins@baker.edu.au.

PMID: 37659140
PMCID: PMC10592019
DOI: 10.1016/j.jdiacomp.2023.108597

Increased intracellular miR-142 in adults with Type 1 diabetes

Li Huang et al. J Diabetes Complications. 2023 Oct.

. 2023 Oct;37(10):108597.

doi: 10.1016/j.jdiacomp.2023.108597. Epub 2023 Aug 25.

Authors

Li Huang¹, Andrzej S Januszewski², Yusuke Takahashi³, David N O'Neal⁴, Jian-Xing Ma⁵, Alicia J Jenkins⁶

Affiliations

¹ Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Department of Ophthalmology, Fujian Medical University Union Hospital, Fuzhou, Fujian Province, China.
² NHMRC Clinical Trials Centre, University of Sydney, Sydney, NSW, Australia; Deptartment of Medicine, University of Melbourne, Melbourne, VIC, Australia; Sydney Pharmacy School, University of Sydney, Sydney, NSW, Australia.
³ Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Deptartment of Biochemistry, Wake Forest University, School of Medicine, Winton-Salem, NC, USA. Electronic address: ytakahas@wakehealth.edu.
⁴ NHMRC Clinical Trials Centre, University of Sydney, Sydney, NSW, Australia; Deptartment of Medicine, University of Melbourne, Melbourne, VIC, Australia; Deptartment of Biochemistry, Wake Forest University, School of Medicine, Winton-Salem, NC, USA.
⁵ Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Deptartment of Biochemistry, Wake Forest University, School of Medicine, Winton-Salem, NC, USA; Baker Heart and Diabetes Institute, Melbourne, VIC, Australia.
⁶ NHMRC Clinical Trials Centre, University of Sydney, Sydney, NSW, Australia; Deptartment of Medicine, University of Melbourne, Melbourne, VIC, Australia; Deptartment of Endocrinology and Diabetes, St. Vincent's Hospital, Melbourne, VIC, Australia; Baker Heart and Diabetes Institute, Melbourne, VIC, Australia. Electronic address: alicia.jenkins@baker.edu.au.

PMID: 37659140
PMCID: PMC10592019
DOI: 10.1016/j.jdiacomp.2023.108597

Abstract

microRNAs (miRs), including miR-142, modulate gene expression and processes implicated in vascular damage and may serve as therapeutic targets and agents, including in Type 1 diabetes (T1D). The project aimed to assess whether miR-142 levels differ between people with and without T1D, and to analyse miR-142 associations with cardiovascular (CVD) risk factors. Intracellular miRs were isolated from whole blood cell pellets using TRIzol-based methodology. In a cross-sectional study in 102 adults cellular miR-142 levels were significantly higher (on unadjusted and adjusted analyses) in 69 adults with T1D relative to 33 non-diabetic subjects: mean ± SD, 3.53 ± 3.66 vs. 1.25 ± 0.78, p < 0.0002, but were not related to HbA1c levels. Further miR-142 research, including longitudinal and intervention studies and basic science are of interest. miR-142 may be valuable in clinical practice for predicting health and as a treatment target.

Keywords: Type 1 diabetes; miR-142; microvascular complications.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

References

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Increased intracellular miR-142 in adults with Type 1 diabetes

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Increased intracellular miR-142 in adults with Type 1 diabetes

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Conflict of interest statement

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